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Product Info Summary
| SKU: | EK2218 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human EPF/HSP10 ELISA Kit PicoKine®
SKU/Catalog Number
EK2218
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human EPF/HSP10 PicoKine ELISA Kit® (96 Tests). Quantitate Human EPF/HSP10 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human EPF/HSP10 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2218)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: M1-D102
Sensitivity
<75 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2218 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2218 is reactive to HSPE1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2218 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of HSP10/EPF
Heat shock 10 kDa protein 1 (Hsp10), also known as chaperonin 10 (cpn10) or early-pregnancy factor (EPF), is a protein that in humans is encoded by the HSPE1 gene. This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2218-CAP | Anti-Human HSPE1 Pre-coated 96-well strip microplate | 1 |
| EK2218-ST | Human HSPE1 Standard | 2 vials, 10 ng/tube |
| EK2218-DA | Human HSPE1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.022 | 0.047 | 0.123 | 0.285 | 0.654 | 0.891 | 1.425 | 1.842 |
Data Example Images
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Human EPF PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human EPF/HSP10 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 108 | 456 | 1755 | 115 | 469 | 1896 |
| Standard deviation | 5.94 | 22.34 | 107.06 | 5.52 | 25.8 | 81.53 |
| CV(%) | 5.5% | 4.9% | 6.1% | 4.8% | 5.5% | 4.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of HSP10/EPF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 108 | 112 | 125 | 101 | 112 | 4.01 | 3.6% |
| Sample 2 | 456 | 496 | 485 | 422 | 465 | 22.77 | 4.9% |
| Sample 3 | 1755 | 1896 | 1744 | 1963 | 1840 | 93.81 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For HSPE1 (Source: Uniprot.Org, NCBI)
Gene Name
HSPE1
Full Name
10 kDa heat shock protein, mitochondrial
Weight
10.932kDa
Superfamily
GroES chaperonin family
Alternative Names
10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; Chaperonin 10 Homolog; Chaperonin 10; CPN10HSP10; Early-pregnancy factor; EPF; GroES; heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1 (chaperonin 10); HSP10; HSPE1 HSPE1 CPN10, EPF, GROES, HSP10 heat shock protein family E (Hsp10) member 1 10 kDa heat shock protein, mitochondrial|10 kDa chaperonin|chaperonin 10|early-pregnancy factor|epididymis secretory sperm binding protein|heat shock 10kD protein 1 (chaperonin 10)|heat shock 10kDa protein 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on HSPE1, check out the HSPE1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for HSPE1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human EPF/HSP10 ELISA Kit PicoKine® (EK2218)
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