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Product Info Summary
| SKU: | EK1410 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human FABP2/I-FABP ELISA Kit PicoKine®
View all FABP2/I-FABP ELISA kits
SKU/Catalog Number
EK1410
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human FABP2/I-FABP ELISA Kit PicoKine™ (96 Tests). Quantitate Human FABP2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FABP2/I-FABP ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1410)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A2-D132
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1410 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1410 is reactive to FABP2 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1410 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FABP2/I-FABP
Fatty acid-binding protein 2 (FABP2), also known as Intestinal-type fatty acid-binding protein (I-FABP) is a protein that in humans is encoded by the FABP2 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. Using a human cDNA probe for in situ hybridization studies, they regionalized the assignment to 4q28-q31. The intestinal form of FABP was mapped to mouse chromosome 3 between the amylase and alcohol dehydrogenase-3 loci. Also, they may be responsible in the modulation of cell growth and proliferation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1410-CAP | Anti-Human FABP2 Pre-coated 96-well strip microplate | 1 |
| EK1410-ST | Human FABP2 Standard | 2 vials, 10 ng/tube |
| EK1410-DA | Human FABP2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.099 | 0.196 | 0.285 | 0.325 | 0.466 | 0.672 | 1.160 | 1.923 |
Data Example Images
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Human FABP2/I-FABP PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FABP2/I-FABP, Dilution ratio of 1:10, concentration in serum and plasma is 1-10 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FABP2/I-FABP ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 49 | 270 | 1038 | 51 | 272 | 977 |
| Standard deviation | 2.69 | 14.31 | 61.24 | 3.57 | 18.76 | 74.25 |
| CV (%) | 5.5% | 5.3% | 5.9% | 7% | 6.9% | 7.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FABP2/I-FABP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 49 | 56 | 59 | 50 | 53 | 4.15 | 7.8% |
| Sample 2 | 270 | 308 | 313 | 281 | 293 | 18.01 | 6.1% |
| Sample 3 | 1038 | 997 | 1031 | 1019 | 1021 | 15.56 | 1.5% |
Protein Target Info & Infographic
Gene/Protein Information For FABP2 (Source: Uniprot.Org, NCBI)
Gene Name
FABP2
Full Name
Fatty acid-binding protein, intestinal
Weight
15.207kDa
Superfamily
calycin superfamily
Alternative Names
FABP2; FABPI; fatty acid binding protein 2, intestinal; Fatty acid-binding protein 2; fatty acid-binding protein, intestinal; IFABP; I-FABP; I-FABPMGC133132; Intestinal FABP; Intestinal-type fatty acid-binding protein FABP2 FABPI, I-FABP fatty acid binding protein 2 fatty acid-binding protein, intestinal|fatty acid binding protein 2, intestinal|intestinal-type fatty acid-binding protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FABP2, check out the FABP2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FABP2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human FABP2/I-FABP ELISA Kit PicoKine® (EK1410)
Hello CJ!
EK1410 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Fecal microbiota transplantation in HIV: A pilot placebo-controlled study
Serrano-Villar S,Talavera-Rodríguez A,Gosalbes MJ,Madrid N,Pérez-Molina JA,Elliott RJ,Navia B,Lanza VF,Vallejo A,Osman M,Dronda F,Budree S,Zamora J,Gutiérrez C,Manzano M,Vivancos MJ,Ron R,Martínez-Sanz J,Herrera S,Ansa U,Moya A,Moreno S. Fecal microbiota
Species: Human
EK1410 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
8 Customer Q&As for Human FABP2/I-FABP ELISA Kit PicoKine®
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of FABP2 in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-07-03
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the FABP2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-07-03
Question
Q: can I use citrate plasma as samples in Human FABP2 Picokine® ELISA Kit (Catalog # EK1410)?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of FABP2 causing disruption of its protein structure. FABP2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the FABP2 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-02-18
Question
Do you have suggestions in handling the blood samples for use with EK1271, EK1410 and EK0694? Can it be stored at room temperature for a day?
Verified customer
Asked: 2019-11-22
Answer
Generally, the collected samples for ELISA analysis can be kept for different periods: 48 hours (2-8°C), 1 month (-20°C) or 6 months (-70°C), but it would be best if you could store them at (2-8°C) for 1 day after collecting the samples with heparin tubes.
Boster Scientific Support
Answered: 2019-11-22
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring FABP2 using the EK1410 Human FABP2 Picokine® ELISA Kit?
W. Garcia
Verified customer
Asked: 2019-10-11
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-10-11
Question
Q: Can FABP2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-01-30
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-01-30
Question
Q: how can I thaw whole blood sample for FABP2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-01-13
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test FABP2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-01-13
Question
Q: how to proceed with the analysis of ELISA data? I have performed FABP2 level in plasma.
Verified Customer
Verified customer
Asked: 2017-01-10
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-01-10
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Anderson
Verified customer
Asked: 2016-02-19
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-02-19
