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Product Info Summary
| SKU: | FEK1257 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human Ficolin-1/FCN1/M Ficolin PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK1257
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Ficolin-1/FCN1/M Ficolin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human FCN1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Ficolin-1/FCN1/M Ficolin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1257)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A30-A326
Sensitivity
<20 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1257 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1257 is reactive to FCN1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin)
Application Guarantee
FEK1257 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Ficolin-1
Ficolin-1, and also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34.3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. Ficolin 1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1257-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1257-ST | Human Ficolin-1 Standard | 2 | 50 ng/tube |
| FEK1257-A | Human FCN1 Antibody A | 1 | 3ml |
| FEK1257-B | Human FCN1 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.003 | 0.177 | 0.313 | 0.558 | 0.926 | 1.491 | 1.972 | 2.357 |
Data Example Images
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Human Ficolin-1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1450 | 8641 | 19842 | 1321 | 8694 | 19651 |
| Standard deviation | 92.8 | 475.26 | 1111.15 | 93.79 | 512.95 | 1061.15 |
| CV (%) | 6.4% | 5.5% | 5.6% | 7.1% | 5.9% | 5.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Ficolin-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1450 | 1365 | 1605 | 1564 | 1496 | 97.24 | 6.5% |
| Sample 2 | 8641 | 7641 | 7712 | 7952 | 7987 | 263.55 | 3.3% |
| Sample 3 | 19842 | 22647 | 21642 | 20458 | 21147 | 1141.95 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For FCN1 (Source: Uniprot.Org, NCBI)
Gene Name
FCN1
Full Name
Ficolin-1
Weight
35.078kDa
Superfamily
ficolin lectin family
Alternative Names
Collagen/fibrinogen domain-containing protein 1; FCN1; Fcna; FCNM; FCNMficolin-A; ficolin (collagen/fibrinogen domain containing) 1; ficolin (collagen/fibrinogen domain-containing) 1; Ficolin1; Ficolin-1; Ficolin-A; ficolin-alpha; M-ficolin FCN1 FCNM ficolin 1 ficolin-1|M-ficolin|ficolin (collagen/fibrinogen domain-containing) 1|ficolin-A|ficolin-alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FCN1, check out the FCN1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FCN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Ficolin-1/FCN1/M Ficolin PicoKine® Quick ELISA Kit (FEK1257)
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