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Product Info Summary
| SKU: | EK2044 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human GAS1 ELISA Kit PicoKine®
SKU/Catalog Number
EK2044
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human GAS1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human GAS1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human GAS1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2044)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L40-S318
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2044 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2044 is reactive to GAS1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2044 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GAS1
Growth arrest-specific protein 1 is a protein that in humans is encoded by the GAS1 gene. This gene is mapped to 9q21.33. Growth arrest-specific 1 plays a role in growth suppression. GAS1 blocks entry to S phase and prevents cycling of normal and transformed cells. Gas1 is a putative tumor suppressor gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2044-CAP | Anti-Human GAS1 Pre-coated 96-well strip microplate | 1 |
| EK2044-ST | Human GAS1 Standard | 2 vials, 2 ng/tube |
| EK2044-DA | Human GAS1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.056 | 0.138 | 0.238 | 0.365 | 0.657 | 1.151 | 1.648 | 2.230 |
Data Example Images
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Human GAS1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect GAS1, Dilution ratio of 1:1, concentration in serum and plasma is around 60 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human GAS1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 55 | 228 | 845 | 60 | 239 | 884 |
| Standard deviation | 2.31 | 14.36 | 38.03 | 3.18 | 15.30 | 63.65 |
| CV (%) | 4.2% | 6.3% | 4.5% | 5.3% | 6.4% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of GAS1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 55 | 53 | 50 | 52 | 52 | 1.80 | 3.4% |
| Sample 2 | 228 | 261 | 228 | 264 | 245 | 17.28 | 7.0% |
| Sample 3 | 845 | 788 | 838 | 845 | 829 | 23.84 | 2.8% |
Protein Target Info & Infographic
Gene/Protein Information For GAS1 (Source: Uniprot.Org, NCBI)
Gene Name
GAS1
Full Name
Growth arrest-specific protein 1
Weight
35.693kDa
Alternative Names
Gas1; GAS-1; growth arrest-specific 1; Growth arrest-specific gene-1; growth arrest-specific protein 1 GAS1 growth arrest specific 1 growth arrest-specific protein 1|GAS-1|Growth arrest-specific gene-1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GAS1, check out the GAS1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GAS1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human GAS1 ELISA Kit PicoKine® (EK2044)
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
5 Customer Q&As for Human GAS1 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-03-03
Question
Q: What is the optimal O.D. value for GAS1 ELISA kit? I performed your GAS1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain GAS1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this GAS1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of GAS1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-08-27
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-03-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-03-22
Question
Q: is it okay to use citrate plasma as samples in Human GAS1 Picokine® ELISA Kit (Catalog # EK2044)?
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of GAS1 causing disruption of its protein structure. GAS1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the GAS1 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-11-23
Question
Q: how can I thaw whole blood sample for GAS1 ELISA after freezing?
D. Dhawan
Verified customer
Asked: 2016-06-18
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test GAS1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2016-06-18
