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Product Info Summary
| SKU: | FEK0991 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human IGFBP7/Igfbp Rp1 PicoKine® Quick ELISA Kit
See all IGFBP-rp1/IGFBP-7 products
SKU/Catalog Number
FEK0991
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IGFBP7/Igfbp Rp1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human IGFBP7/Igfbp Rp1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 20 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IGFBP7/Igfbp Rp1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0991)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: D30-L282
Sensitivity
<300 pg/ml
Assay Range
625 pg/ml - 40,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0991 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0991 is reactive to IGFBP7 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine
Application Guarantee
FEK0991 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IGFBP-rp1/IGFBP-7
Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors(IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0991-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0991-ST | Human IGFBP-rp1/IGFBP-7 Standard | 2 | 40 ng/tube |
| FEK0991-A | Human IGFBP7 Antibody A | 1 | 3ml |
| FEK0991-B | Human IGFBP7 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 | 40,000 |
| O.D. | 0.029 | 0.092 | 0.143 | 0.237 | 0.443 | 0.848 | 1.570 | 2.243 |
Data Example Images
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Human IGFBP7 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1401 | 2986 | 17423 | 1521 | 3364 | 17532 |
| Standard deviation | 60.24 | 134.37 | 731.77 | 88.22 | 161.47 | 806.47 |
| CV(%) | 4.3% | 4.5% | 4.2% | 5.8% | 4.8% | 4.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IGFBP-rp1/IGFBP-7 in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1401 | 1416 | 1369 | 1426 | 1403 | 44.90 | 3.2% |
| Sample 2 | 2986 | 3531 | 3163 | 3154 | 3209 | 144.38 | 4.5% |
| Sample 3 | 17423 | 15436 | 16428 | 16654 | 16485 | 560.5 | 3.4% |
Protein Target Info & Infographic
Gene/Protein Information For IGFBP7 (Source: Uniprot.Org, NCBI)
Gene Name
IGFBP7
Full Name
Insulin-like growth factor-binding protein 7
Weight
29.13kDa
Alternative Names
angiomodulin; FSTL2; IBP-7; IGFBP7; IGFBP-7; IGFBP-7PGI2-stimulating factor; IGFBP-7v; IGFBPrp1; IGFBP-rp1; insulin-like growth factor binding protein 7; insulin-like growth factor-binding protein 7; MAC25 protein; Mac25; MAC25IGF-binding protein 7; Prostacyclin-stimulating factor; PSF; PSFAGM; TAF; Tumor-derived adhesion factor IGFBP7 AGM, FSTL2, IBP-7, IGFBP-7, IGFBP-7v, IGFBPRP1, MAC25, PSF, RAMSVPS, TAF insulin like growth factor binding protein 7 insulin-like growth factor-binding protein 7|IGF-binding protein 7|IGFBP-rP1|PGI2-stimulating factor|angiomodulin|prostacyclin-stimulating factor|tumor-derived adhesion factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IGFBP7, check out the IGFBP7 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IGFBP7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IGFBP7/Igfbp Rp1 PicoKine® Quick ELISA Kit (FEK0991)
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