Click image to see more details
Product Info Summary
| SKU: | M02410 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IGFBP7 Monoclonal Antibody
SKU/Catalog Number
M02410
BM5547 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IGFBP7 Monoclonal Antibody catalog # M02410. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IGFBP7 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02410)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEDO-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IGFBP7
Reactive Species
M02410 is reactive to IGFBP7 in Human, Mouse, Rat
Observed Molecular Weight
29 kDa
Calculated molecular weight
29.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02410 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:200
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:5k dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of IGFBP7 expression in SW480 cell lysate.
Click image to see more details
IHC analysis of IGFBP7 using anti-IGFBP7 antibody (M02410).
IGFBP7 was detected in a paraffin-embedded section of human pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IGFBP7 Antibody (M02410) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-IGFBP7 Monoclonal Antibody (M02410)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IGFBP7 Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-IGFBP7 Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-IGFBP7 Monoclonal Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for urinary bladder carcinoma using anti-IGFBP7 Monoclonal antibody M02410. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-25
Question
What sample was tested in WB and IHC applications with M02410?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of IGFBP7 expression in SW480 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Will anti-IGFBP7 Monoclonal antibody M02410 work on goat WB with leptomeninges?
Verified Customer
Verified customer
Asked: 2017-11-28
Answer
Our lab technicians have not tested anti-IGFBP7 Monoclonal antibody M02410 on goat. You can run a BLAST between goat and the immunogen sequence of anti-IGFBP7 Monoclonal antibody M02410 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat leptomeninges in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-28
Question
Is there a BSA free version of anti-IGFBP7 Monoclonal antibody M02410 available?
A. Anderson
Verified customer
Asked: 2016-12-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IGFBP7 Monoclonal antibody M02410 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-12-30
Question
We are currently using anti-IGFBP7 Monoclonal antibody M02410 for rat tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
W. Zhang
Verified customer
Asked: 2015-04-30
Answer
The anti-IGFBP7 Monoclonal antibody (M02410) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-30
Question
I was wanting to use your anti-IGFBP7 Monoclonal antibody for WB for human urinary bladder carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human urinary bladder carcinoma identification?
B. Miller
Verified customer
Asked: 2015-03-19
Answer
You can see on the product datasheet, M02410 anti-IGFBP7 Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human urinary bladder carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-19
