Click image to see more details
Product Info Summary
| SKU: | EK1365 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human IL-34/Interleukin-34 ELISA Kit PicoKine®
SKU/Catalog Number
EK1365
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-34 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL34 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-34/Interleukin-34 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1365)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: N21-P242
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1365 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1365 is reactive to Il34 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1365 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-34
Interleukin-34, or IL-34 is a protein belonging to a group of cytokines called interleukins. It is mapped to 16q22.1. The discovery of IL-34 protein in the red pulp of the spleen suggests involvement in growth and development of myeloid cells, consistent with its activity on monocytes. IL-34 is a recently discovered cytokine that acts on tissue resident macrophages and Langerhans cells upon binding the receptor for CSF-1, CSF-1R. It also can promote the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor. In addition, it promotes the development, survival, and function of microglia and Langerhans cells, therefore, this cytokine may predominately function in brain and skin biology.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1365-CAP | Anti-Human Il34 Pre-coated 96-well strip microplate | 1 |
| EK1365-ST | Human Il34 Standard | 2 vials, 10 ng/tube |
| EK1365-DA | Human Il34 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.017 | 0.088 | 0.151 | 0.258 | 0.458 | 0.817 | 1.395 | 2.004 |
Data Example Images
Click image to see more details
Human IL-34 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-34, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 62.5 pg/ml..
Some articles we found to cite concentrations of IL-34 in samples: 22264405 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-34/Interleukin-34 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 93 | 548 | 1157 | 96 | 552 | 1148 |
| Standard deviation | 5.3 | 33.42 | 67.1 | 5.85 | 35.32 | 73.47 |
| CV (%) | 5.7% | 6.1% | 5.8% | 6.1% | 6.4% | 6.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-34 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 93 | 84 | 90 | 91 | 89 | 3.35 | 3.7% |
| Sample 2 | 548 | 521 | 516 | 487 | 518 | 21.64 | 4.1% |
| Sample 3 | 1157 | 1352 | 1188 | 1232 | 1232 | 74.09 | 6% |
Protein Target Info & Infographic
Gene/Protein Information For Il34 (Source: Uniprot.Org, NCBI)
Gene Name
Il34
Full Name
Interleukin-34
Weight
26.764kDa
Superfamily
IL-34 family
Alternative Names
C16orf77; IL34; IL-34; interleukin 34 Il34|2010004A03Rik, AI593503|interleukin 34|interleukin-34
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Il34, check out the Il34 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il34: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL-34/Interleukin-34 ELISA Kit PicoKine® (EK1365)
Hello CJ!
No publications found for EK1365
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human IL-34/Interleukin-34 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Human IL-34/Interleukin-34 ELISA Kit PicoKine®
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Human IL-34/Interleukin-34 ELISA Kit PicoKine®
Question
Q: is it okay to use citrate plasma as samples in Human IL-34 Picokine® ELISA Kit (Catalog # EK1365)?
P. Jackson
Verified customer
Asked: 2020-03-29
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of IL-34 causing degradation of its protein structure. IL-34 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the IL-34 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-03-29
Question
Q: What is the optimal O.D. value for IL-34 ELISA kit? I performed your IL-34 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-34 even though the O.D. values are not very high?
C. Dhawan
Verified customer
Asked: 2020-02-02
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-34 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of IL-34 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-02-02
Question
Q: if the enzyme conjugated IL-34 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-34 antigen?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-07-18
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-09-16
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-09-16
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
D. Walker
Verified customer
Asked: 2018-04-25
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-04-25
Question
Q: which procedure should I follow in order to thaw whole blood sample for IL-34 ELISA after freezing?
C. Perez
Verified customer
Asked: 2018-02-13
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-34 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-02-13
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2015-08-15
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2015-08-15
