Product Info Summary
SKU: | FEK0407 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Assay Time: | 120 Minutes |
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Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Human IL-5/Interleukin-5 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0407
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-5/Interleukin-5 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human IL5 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-5/Interleukin-5 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0407)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: I20-S134
Sensitivity
<2 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0407 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0407 is reactive to IL5 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
FEK0407 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-5
Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.
Kit Components
Catalog number | Description | Quantity |
---|---|---|
FEK0407-CAP | 96-well plate precoated with anti-Human IL-5 antibody | 1 |
EK0407-ST | lyophilized recombinant Human IL-5 standard | 10 ng/tube |
FEK0407-DA | Biotinylated anti-Human IL-5 detection antibody (50x) | 100 ul |
- | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
AR1106-1 | Sample diluent | 30 ml |
AR1106-2 | Antibody diluent | 12 ml |
- | Avidin-Biotin-Peroxidase Diluent | 12 ml |
AR1106-5 | Wash Buffer (25x) | 20 ml |
AR1104 | Color Developing Reagent (TMB) | 10 ml |
AR1105 | Stop Solution | 10 ml |
PLA-SEA | Plate Sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
O.D. | 0.005 | 0.078 | 0.150 | 0.269 | 0.501 | 0.862 | 1.545 | 2.201 |
Data Example Images
Click image to see more details
Human IL-5 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 9 | 73 | 238 | 8 | 70 | 234 |
Standard deviation | 0.7 | 4.67 | 16.42 | 0.74 | 4.55 | 20.12 |
CV (%) | 7.8% | 6.4% | 6.9% | 9.3% | 6.5% | 8.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-5 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 9 | 10 | 10 | 9 | 9 | 0.5 | 5.5% |
Sample 2 | 73 | 73 | 74 | 62 | 70 | 4.92 | 7% |
Sample 3 | 238 | 246 | 219 | 235 | 234 | 9.81 | 4.1% |
Protein Target Info & Infographic
Gene/Protein Information For IL5 (Source: Uniprot.Org, NCBI)
Uniprot ID
P05113
Gene ID
3567
Gene Name
IL5
Full Name
Interleukin-5
Weight
15.238kDa
Superfamily
IL-5 family
Alternative Names
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I IL5 EDF, IL-5, TRF interleukin 5 interleukin-5|B-cell differentiation factor I|T-cell replacing factor|colony-stimulating factor, eosinophil|eosinophil differentiation factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL5, check out the IL5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL-5/Interleukin-5 PicoKine® Quick ELISA Kit (FEK0407)
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