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Product Info Summary
| SKU: | EK2247 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human Myocilin ELISA Kit PicoKine®
SKU/Catalog Number
EK2247
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Myocilin PicoKine ELISA Kit® (96 Tests). Quantitate Human Myocilin in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Myocilin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2247)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: HEK293; Immunogen sequence: L215-M504
Sensitivity
<75 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2247 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2247 is reactive to MYOC in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2247 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Myocilin
Myocilin, trabecular meshwork inducible glucocorticoid response (TIGR), also known as MYOC, is a protein which in humans is encoded by the MYOC gene. MYOC encodes the protein myocilin, which is believed to have a role in cytoskeletal function. MYOC is expressed in many occular tissues, including the trabecular meshwork, and was revealed to be the trabecular meshwork glucocorticoid-inducible response protein (TIGR). The trabecular meshwork is a specialized eye tissue essential in regulating intraocular pressure, and mutations in MYOC have been identified as the cause of hereditary juvenile-onset open-angle glaucoma.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2247-CAP | Anti-Human MYOC Pre-coated 96-well strip microplate | 1 |
| EK2247-ST | Human MYOC Standard | 2 vials, 10 ng/tube |
| EK2247-DA | Human MYOC Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.026 | 0.043 | 0.086 | 0.231 | 0.568 | 1.085 | 1.523 | 2.002 |
Data Example Images
Click image to see more details
Human Myocilin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myocilin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 270 | 550 | 2677 | 221 | 494 | 4349 |
| Standard deviation | 8.1 | 21.51 | 55.41 | 7.67 | 27.57 | 277.9 |
| CV(%) | 3% | 3.9% | 2.1% | 3.5% | 5.6% | 6.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Myocilin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 270 | 223 | 195 | 223 | 228 | 7.27 | 3.2% |
| Sample 2 | 550 | 595 | 615 | 586 | 587 | 33.78 | 5.8% |
| Sample 3 | 2677 | 2970 | 4384 | 4862 | 3723 | 103.88 | 2.8% |
Protein Target Info & Infographic
Gene/Protein Information For MYOC (Source: Uniprot.Org, NCBI)
Gene Name
MYOC
Full Name
Myocilin
Weight
Alternative Names
GLC1A; GLC1Amyocilin; GPOA; JOAG; JOAG1; MYOC; Myocilin; myocilin, trabecular meshwork inducible glucocorticoid response; TIGR; TIGRmutated trabecular meshwork-induced glucocorticoid response protein; Trabecular meshwork-induced glucocorticoid response protein MYOC GLC1A, GPOA, JOAG, JOAG1, TIGR myocilin myocilin|juvenile-onset open-angle glaucoma 1|mutated trabecular meshwork-induced glucocorticoid response protein|myocilin 55 kDa subunit|myocilin trabecular meshwork inducible glucocorticoid response protein|trabecular meshwork inducible glucocorticoid response protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MYOC, check out the MYOC Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MYOC: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Myocilin ELISA Kit PicoKine® (EK2247)
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