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Product Info Summary
| SKU: | EK1531 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Human Pan Trypsin ELISA Kit PicoKine®
View all Trypsin 2/PRSS2 ELISA kits
SKU/Catalog Number
EK1531
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Pan Trypsin ELISA Kit PicoKine™ (96 Tests). Quantitate Human Pan Trypsin in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Pan Trypsin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1531)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A16-S247
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1531 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1531 is reactive to PRSS2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK1531 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Trypsin 2/PRSS2
Trypsin is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyses proteins. In the duodenum, trypsin catalyzes the hydrolysis of peptide bonds, breaking down proteins into smaller peptides. The peptide products are then further hydrolyzed into amino acids via other proteases, rendering them available for absorption into the blood stream. Tryptic digestion is a necessary step in protein absorption as proteins are generally too large to be absorbed through the lining of the small intestine. Additionally, Trypsin is produced as the inactive zymogen trypsinogen in the pancreas. When the pancreas is stimulated by cholecystokinin, it is then secreted into the first part of the small intestine (the duodenum) via the pancreatic duct. Once in the small intestine, the enzyme enteropeptidase activates trypsinogen into trypsin by proteolytic cleavage. Auto catalysis can happen with trypsin using trypsinogen as the substrate. This activation mechanism is common for most serine proteases, and serves to prevent autodegradation of the pancreas.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1531-CAP | Anti-Human PRSS2 Pre-coated 96-well strip microplate | 1 |
| EK1531-ST | Human PRSS2 Standard | 2 vials, 10 ng/tube |
| EK1531-DA | Human PRSS2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.117 | 0.202 | 0.249 | 0.324 | 0.571 | 0.937 | 1.516 | 2.023 |
Data Example Images
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Human Pan Trypsin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Trypsin 2/PRSS2, Dilution ratio of 1:200, concentration in serum and plasma is around 180 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Pan Trypsin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 85 | 261 | 887 | 82 | 235 | 820 |
| Standard deviation | 6.03 | 20.09 | 66.52 | 6.88 | 22.32 | 64.78 |
| CV (%) | 7.1% | 7.7% | 7.5% | 8.4% | 9.5% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Trypsin 2/PRSS2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 85 | 100 | 85 | 91 | 90 | 6.13 | 6.8% |
| Sample 2 | 261 | 265 | 235 | 246 | 251 | 11.98 | 4.7% |
| Sample 3 | 887 | 951 | 881 | 978 | 924 | 41.42 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For PRSS2 (Source: Uniprot.Org, NCBI)
Gene Name
PRSS2
Full Name
Trypsin-2
Weight
26.488kDa
Superfamily
peptidase S1 family
Alternative Names
Anionic trypsinogen; EC 3.4.21; EC 3.4.21.4; MGC111183; MGC120174; MGC120175; protease serine 2 preproprotein; protease, serine, 2 (trypsin 2); protease, serine, 2, preproprotein; PRSS2; Serine protease 2; Tesp4; TRY2TRY8; TRY8; TRYP2; Trypsin 2; Trypsin II; trypsin-2; trypsinogen 2; Trypsinogen 20 PRSS2 TRY2, TRY8, TRYP2 serine protease 2 trypsin-2|anionic trypsinogen|protease serine 2 preproprotein|protease, serine 2|protease, serine, 2 (trypsin 2)|trypsin II|trypsinogen 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PRSS2, check out the PRSS2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PRSS2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Pan Trypsin ELISA Kit PicoKine® (EK1531)
Hello CJ!
EK1531 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Comprehensive Proteomic Profiling of Patients’ Tears Identifies Potential Biomarkers for the Traumatic Vegetative State
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human Pan Trypsin ELISA Kit PicoKine®
Question
Does EK1531 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Human Pan Trypsin(PRSS1/2/3) ELISA Kit PicoKine® (EK1531) doesn't contain native human biological materials. doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Does EK1531 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Human Pan Trypsin(PRSS1/2/3) ELISA Kit PicoKine® (EK1531) doesn't contain native human biological materials. doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Is EK1531 only for “Human PRSS3” or “Human Pan Trypsin(PRSS1/2/3)”?
Verified customer
Asked: 2021-02-22
Answer
The Human Pan Trypsin(PRSS1/2/3) ELISA Kit PicoKine™ (EK1531) is for Human Pan Trypsin(PRSS1/2/3).
Boster Scientific Support
Answered: 2021-02-23
Question
Q: how to analyze ELISA data? I measured Trypsin 3 level in plasma.
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-03-13
Question
Q: how to thaw whole blood sample for Trypsin 3 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Trypsin 3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-01-03
Question
Q: What is the optimal O.D. value for Trypsin 3 ELISA kit? I used your Trypsin 3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Trypsin 3 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Trypsin 3 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Trypsin 3 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-10-09
Question
Q: if the enzyme conjugated Trypsin 3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Trypsin 3 antigen?
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-05-10
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
L. Krishna
Verified customer
Asked: 2018-03-07
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-03-07
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Trypsin 3 using the EK1531 Human Trypsin 3 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-04-11
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2017-04-11
