Product Info Summary
SKU: | EK1712 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine®
View all Serpin F2/alpha 2-Antiplasmin ELISA kits
SKU/Catalog Number
EK1712
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human SERPINF2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml .
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1712)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: N40-K491
Sensitivity
<10 pg/ml
Assay Range
1.56 ng/ml - 100 ng/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1712 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1712 is reactive to SERPINF2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK1712 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Serpin F2/alpha 2-Antiplasmin
Human SerpinF2 encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK1712-CAP | Anti-Human SERPINF2 Pre-coated 96-well strip microplate | 1 |
EK1712-ST | Human SERPINF2 Standard | 2 vials, 100 ng/tube |
EK1712-DA | Human SERPINF2 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (ng/ml) | 0 | 1.56 | 3.13 | 6.25 | 12.5 | 25 | 50 | 100 |
O.D. | 0.074 | 0.253 | 0.335 | 0.536 | 0.815 | 1.219 | 1.671 | 1.915 |
Data Example Images
Click image to see more details
Human SerpinF2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Serpin F2/alpha 2-Antiplasmin, Dilution ratio of 1:1000, concentration in serum and plasma is around 15 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 1883 | 10402 | 40754 | 1930 | 12110 | 43978 |
Standard deviation | 114.86 | 488.89 | 2282.2 | 123.52 | 884.03 | 3430.28 |
CV (%) | 6.1% | 4.7% | 5.6% | 6.4% | 7.3% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Serpin F2/alpha 2-Antiplasmin in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 1883 | 2175 | 2014 | 2130 | 2050 | 113.14 | 5.5% |
Sample . 2 | 10402 | 11339 | 11464 | 10592 | 10949 | 459.34 | 4.1%% |
Sample 3 | 40754 | 39524 | 41038 | 45998 | 41828 | 2473.60 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For SERPINF2 (Source: Uniprot.Org, NCBI)
Gene Name
SERPINF2
Full Name
Alpha-2-antiplasmin
Weight
54.566kDa
Superfamily
serpin family
Alternative Names
AAPclade F (alpha-2 antiplasmin; alpha 2-Antiplasmin; Alpha-2-AP; Alpha-2-PI; Alpha-2-plasmin inhibitor; pigment epithelium derived factor), member 2; Serpin F2; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 2 SERPINF2 A2AP, AAP, ALPHA-2-PI, API, PLI, alpha2AP serpin family F member 2 alpha-2-antiplasmin|alpha-2-AP|alpha-2-plasmin inhibitor|plasmin inhibitor|serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2|serpin F2|serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SERPINF2, check out the SERPINF2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SERPINF2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine® (EK1712)
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No publications found for EK1712
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Customer Q&As
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Find answers in Q&As, reviews.
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5 Customer Q&As for Human Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine®
Question
Q: how to thaw whole blood sample for Serpin F2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-07-13
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Serpin F2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-07-13
Question
Q: is it okay to use EDTA plasma as samples in Human Serpin F2 Picokine® ELISA Kit (Catalog # EK1712)?
P. Campbell
Verified customer
Asked: 2019-02-05
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Serpin F2 causing disruption of its protein structure. Serpin F2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Serpin F2 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-02-05
Question
Q: What is the optimal O.D. value for Serpin F2 ELISA kit? I performed your Serpin F2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Serpin F2 even though the O.D. values are not very high?
R. Green
Verified customer
Asked: 2018-07-31
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Serpin F2 ELISA kit to have sensitivity of 10pg/ml , that means the minimum amount of Serpin F2 that can be declared/interpreted as positive by the above standard is 10pg/ml .
Boster Scientific Support
Answered: 2018-07-31
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Serpin F2 in Human plasma ? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
T. Mehta
Verified customer
Asked: 2018-07-20
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Serpin F2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-07-20
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Serpin F2 using the EK1712 Human Serpin F2 Picokine® ELISA Kit?
Y. Anand
Verified customer
Asked: 2014-04-03
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2014-04-03