Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine™

Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Serpinf2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml .

Product Info Summary

SKU: EK1713
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine™

See all Serpin F2/alpha 2-Antiplasmin products

SKU/Catalog Number

EK1713

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Serpinf2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml .

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1713)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: N40-K491

Sensitivity

<10 pg/ml

Assay Range

1.56 ng/ml - 100 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1713 is reactive to Serpinf2 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK1713 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1713-CAP Anti-Mouse Serpinf2 Pre-coated 96-well strip microplate 1
EK1713-ST Mouse Serpinf2 Standard 2 vials, 100 ng/tube
EK1713-DA Mouse Serpinf2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)01.563.136.2512.52550100
O.D.0.0660.1220.1750.2760.5200.9521.4082.380

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Serpin F2/alpha 2-Antiplasmin, Dilution ratio of 1:500, concentration in serum is around 7.5ug/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2710138644237629001420045464
Standard deviation113.82804.12712.06130.5965.63273.4
CV (%)4.2%5.8%6.4%4.5%6.8%7.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Serpin F2/alpha 2-Antiplasmin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 127102230270622772480227.869.1%
Sample . 21386412175133801210512881760.905.9%%
Sample 342376429474756746317448012194.704.8%
*number of samples for each test n=16.

Gene/Protein Information For Serpinf2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q61247

Gene ID

18816

Gene Name

Serpinf2

Full Name

Alpha-2-antiplasmin

Weight

54.972kDa

Superfamily

serpin family

Alternative Names

AAPclade F (alpha-2 antiplasmin; alpha 2-Antiplasmin; Alpha-2-AP; Alpha-2-PI; Alpha-2-plasmin inhibitor; pigment epithelium derived factor), member 2; Serpin F2; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 2 Serpinf2|AI747498, Pli, Serpimf2|serine (or cysteine) peptidase inhibitor, clade F, member 2|alpha-2-antiplasmin|alpha-2-AP|alpha-2-PI|alpha-2-plasmin inhibitor|pigment epithelium derived factor|plasmin inhibitor alpha 2|serine (or cysteine) proteinase inhibitor, clade F, member 2|serpin F2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Serpinf2, check out the Serpinf2 Infographic

Serpinf2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Serpinf2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

D. Chakrabarti

Verified customer

Asked: 2019-07-10

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-07-10

Question

Q: if the enzyme conjugated Serpin F2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Serpin F2 antigen?

J. Phillips

Verified customer

Asked: 2018-06-24

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-06-24

Question

Q: What is the optimal O.D. value for Serpin F2 ELISA kit? I used your Serpin F2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Serpin F2 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-05-11

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Serpin F2 ELISA kit to have sensitivity of 10pg/ml , that means the minimum amount of Serpin F2 that can be declared/interpreted as positive by the above standard is 10pg/ml .

Boster Scientific Support

Answered: 2018-05-11

Question

Q: how to analyze ELISA data? I have performed Serpin F2 level in plasma .

Verified Customer

Verified customer

Asked: 2018-05-01

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-05-01

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Serpin F2 using the EK1713 Mouse Serpin F2 Picokine® ELISA Kit?

Z. Li

Verified customer

Asked: 2017-05-09

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2017-05-09

Question

Q: how to thaw whole blood sample for Serpin F2 ELISA after freezing?

R. Patel

Verified customer

Asked: 2017-02-15

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Serpin F2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2017-02-15

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