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Product Info Summary
| SKU: | EK1202 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human beta IG-H3/TGFBI ELISA Kit PicoKine®
View all Beta Ig-h3/TGFBI ELISA kits
SKU/Catalog Number
EK1202
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human beta IG-H3/TGFBI ELISA Kit PicoKine™ (96 Tests). Quantitate Human TGFBI in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human beta IG-H3/TGFBI ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1202)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: G24-H683
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1202 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1202 is reactive to TGFBI in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1202 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Beta Ig-h3/TGFBI
Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1202-CAP | Anti-Human TGFBI Pre-coated 96-well strip microplate | 1 |
| EK1202-ST | Human TGFBI Standard | 2 vials, 10 ng/tube |
| EK1202-DA | Human TGFBI Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.044 | 0.068 | 0.107 | 0.173 | 0.353 | 0.674 | 1.198 | 2.028 |
Data Example Images
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Human TGFBI/beta IG-H3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human beta IG-H3/TGFBI ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 154 | 388 | 1132 | 160 | 387 | 1210 |
| Standard deviation | 6.77 | 28.32 | 57.73 | 7.36 | 34.41 | 70.18 |
| CV (%) | 4.4% | 7.3% | 5.8% | 4.6% | 9.1% | 5.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Beta Ig-h3/TGFBI in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 154 | 145 | 147 | 157 | 150 | 4.91 | 3.2% |
| Sample 2 | 388 | 396 | 437 | 439 | 415 | 23.18 | 5.5% |
| Sample 3 | 1132 | 1229 | 1284 | 1106 | 1187 | 72.03 | 6% |
Protein Target Info & Infographic
Gene/Protein Information For TGFBI (Source: Uniprot.Org, NCBI)
Gene Name
TGFBI
Full Name
Transforming growth factor-beta-induced protein ig-h3
Weight
74.681kDa
Alternative Names
beta IGH3; beta IG-H3; BIGH3EBMD; CDB1; CDG2; CDGG1transforming growth factor, beta-induced, 68kD; CSD; CSD1; CSD2; CSD3; Kerato-epithelin; LCD1; RGD-CAP; RGD-containing collagen-associated protein; TGFBI; TGFBIP; transforming growth factor, beta-induced, 68kDa; transforming growth factor-beta-induced protein ig-h3 TGFBI BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, LCD1 transforming growth factor beta induced transforming growth factor-beta-induced protein ig-h3|RGD-CAP|RGD-containing collagen-associated protein|beta ig-h3|betaig-h3|kerato-epithelin|transforming growth factor beta-induced 68kDa|transforming growth factor, beta-induced, 68kD
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TGFBI, check out the TGFBI Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TGFBI: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human beta IG-H3/TGFBI ELISA Kit PicoKine® (EK1202)
Hello CJ!
EK1202 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jin Y, Wan Y, Chen G, Chen L, Zhang Mq, Deng L, Zhang Jc, Xiong Xz, Xin Jb. Plos One. 2014 Oct 16;9(10):E111044. Doi: 10.1371/Journal.Pone.0111044. Ecollection 2014. Treg/Il-17 Ratio And Treg Differentiation In Patients With Copd.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
10 Customer Q&As for Human beta IG-H3/TGFBI ELISA Kit PicoKine®
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Beta Ig-h3 using the EK1202 Human Beta Ig-h3 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-11-19
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2020-11-19
Question
Q: can you suggest the dilution ratio of serum samples for detection of Beta Ig-h3 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
H. White
Verified customer
Asked: 2020-06-05
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Beta Ig-h3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-06-05
Question
Q: What is the optimal O.D. value for Beta Ig-h3 ELISA kit? I used your Beta Ig-h3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Beta Ig-h3 even though the O.D. values are not very high?
C. Martinez
Verified customer
Asked: 2020-02-07
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Beta Ig-h3 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Beta Ig-h3 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-02-07
Question
Would AR1106-1 be suitable as a diluent for EK1202? Would it be fine to dilute the samples into Phosphate-buffered saline for early steps and then use a sample diluent buffer for the last dilution step?
Verified customer
Asked: 2019-11-21
Answer
Yes, a Sample Diluent Buffer (AR1106-1) is suitable to be a diluent for Human beta IG-H3/TGFBI ELISA Kit PicoKine. It would be okay to dilute the samples into PBS for the early steps and into the sample diluent from the kit for the last dilution step.
Boster Scientific Support
Answered: 2019-11-22
Question
Would AR1106-1 be suitable as a diluent for Human beta IG-H3/TGFBI ELISA Kit PicoKine EK1202?
Verified customer
Asked: 2019-11-21
Answer
Yes, AR1106-1 Sample Diluent Buffer is suitable to be a diluent for EK1202.
Boster Scientific Support
Answered: 2019-11-22
Question
Q: if the enzyme conjugated Beta Ig-h3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Beta Ig-h3 antigen?
S. Kaur
Verified customer
Asked: 2019-07-28
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-07-28
Question
Q: can I use citrate plasma as samples in Human Beta Ig-h3 Picokine® ELISA Kit (Catalog # EK1202)?
Verified Customer
Verified customer
Asked: 2018-09-25
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Beta Ig-h3 causing disruption of its protein structure. Beta Ig-h3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Beta Ig-h3 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-09-25
Question
Q: Can Beta Ig-h3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
G. Jones
Verified customer
Asked: 2018-09-03
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-09-03
Question
Q: how to analyze ELISA data? I have performed Beta Ig-h3 level in serum.
V. Mitchell
Verified customer
Asked: 2017-05-31
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-05-31
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
M. Adams
Verified customer
Asked: 2015-09-07
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-09-07
