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Product Info Summary
| SKU: | M01218-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TGFBI/Beta Ig H3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01218-2
BM3901 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TGFBI/Beta Ig H3 Rabbit Monoclonal Antibody catalog # M01218-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TGFBI/Beta Ig H3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01218-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DI-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TGFBI
Reactive Species
M01218-2 is reactive to TGFBI in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
74.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01218-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: human U251 whole cell, human Hela whole cell,
IHC: human kideny tissue
Validation Images & Assay Conditions
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Western blot analysis of TGFB1 using anti-TGFB1 antibody (M01218-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGFB1 antigen affinity purified monoclonal antibody (M01218-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TGFB1 at approximately 68 kDa. The expected band size for TGFB1 is at 75 kDa.
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Immunohistochemical analysis of paraffin-embedded human kideny, using TGFBI Antibody.
Specific Publications For Anti-TGFBI/Beta Ig H3 Rabbit Monoclonal Antibody (M01218-2)
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6 Customer Q&As for Anti-TGFBI/Beta Ig H3 Rabbit Monoclonal Antibody
Question
Is this M01218-2 anti-TGFBI/Beta Ig H3 Rabbit Monoclonal antibody reactive to the isotypes of TGFBI?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
The immunogen of M01218-2 anti-TGFBI/Beta Ig H3 Rabbit Monoclonal antibody is A synthesized peptide derived from human TGFBI. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-05
Question
We are currently using anti-TGFBI/Beta Ig H3 Rabbit Monoclonal antibody M01218-2 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
The anti-TGFBI/Beta Ig H3 Rabbit Monoclonal antibody (M01218-2) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-25
Question
Is a blocking peptide available for product anti-TGFBI/Beta Ig H3 Rabbit Monoclonal antibody (M01218-2)?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
We do provide the blocking peptide for product anti-TGFBI/Beta Ig H3 Rabbit Monoclonal antibody (M01218-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-21
Question
Does anti-TGFBI/Beta Ig H3 Rabbit Monoclonal antibody M01218-2 work for IHC with liver?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
According to the expression profile of liver, TGFBI is highly expressed in liver. So, it is likely that anti-TGFBI/Beta Ig H3 Rabbit Monoclonal antibody M01218-2 will work for IHC with liver.
Boster Scientific Support
Answered: 2019-05-23
Question
I was wanting to use your anti-TGFBI/Beta Ig H3 Rabbit Monoclonal antibody for IHC for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
E. Johnson
Verified customer
Asked: 2018-07-05
Answer
It shows on the product datasheet, M01218-2 anti-TGFBI/Beta Ig H3 Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-05
Question
I would like to test anti-TGFBI/Beta Ig H3 Rabbit Monoclonal antibody M01218-2 on human liver for research purposes, then I may be interested in using anti-TGFBI/Beta Ig H3 Rabbit Monoclonal antibody M01218-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-23
Answer
The products we sell, including anti-TGFBI/Beta Ig H3 Rabbit Monoclonal antibody M01218-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-23
