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Product Info Summary
| SKU: | LS027178 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of interleukin 1 family, member 7 (zeta) (IL1F7), transcript variant 1 |
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Product info
Product Name
IL37 (NM_014439) Human Over-expression Lysate
SKU/Catalog Number
LS027178
Size
100 µg
Description
Transient overexpression lysate of interleukin 1 family, member 7 (zeta) (IL1F7), transcript variant 1
Storage & Handling
Cite This Product
IL37 (NM_014439) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS027178)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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IL37 Human Over-expression Lysates NM_014439.
Western blot validation of overexpression lysate (LS027178) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IL37 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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IL37 Human Over-expression Lysates NM_173203.
Western blot validation of overexpression lysate (LS027178) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with IL37 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for IL37 (Source: Uniprot.org, NCBI)
Gene Name
IL37
Full Name
Interleukin-37
Weight
24.126kDa
Superfamily
IL-1 family
Alternative Names
FIL1 zeta; FIL1; FIL1(ZETA); FIL1ZFIL1 zeta; IL-1F7; IL-1F7IL-1 zeta; IL1H4IL1F7 (canonical product IL-1F7b); IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1); IL-1RP1IL-1X protein; IL1RP1interleukin 1 family member 7; IL-1X; IL37; IL-37; interleukin 1 family, member 7 (zeta); interleukin 1, zeta; interleukin-1 family member 7; Interleukin-1 homolog 4; interleukin-1 superfamily z; Interleukin-1 zeta; Interleukin-1-related protein IL37 FIL1, FIL1(ZETA), FIL1Z, IL-1F7, IL-1H, IL-1H4, IL-1RP1, IL-23, IL-37, IL1F7, IL1H4, IL1RP1 interleukin 37 interleukin-37|FIL1 zeta|IL-1 zeta|IL-1F7b (IL-1H4, IL-1H, IL-1RP1)|IL-1X protein|IL1F7 (canonical product IL-1F7b)|interleukin 1 family member 7|interleukin 1, zeta|interleukin-1 homolog 4|interleukin-1 superfamily z|interleukin-1-related protein|interleukin-23
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on IL37, check out the IL37 Infographic
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In this infographic, you will see the following information for IL37: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For IL37 (NM_014439) Human Over-expression Lysate (LS027178)
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