Product Info Summary
SKU: | LS026173 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of integrator complex subunit 1 (INTS1) |
Product info
Product Name
INTS1 (NM_001080453) Human Over-expression Lysate
SKU/Catalog Number
LS026173
Size
100 µg
Description
Transient overexpression lysate of integrator complex subunit 1 (INTS1)
Storage & Handling
Cite This Product
INTS1 (NM_001080453) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS026173)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Protein Target Info & Infographic
Gene/Protein Information for INTS1 (Source: Uniprot.org, NCBI)
Gene Name
INTS1
Full Name
Integrator complex subunit 1
Weight
244.297kDa
Superfamily
Integrator subunit 1 family
Alternative Names
DKFZp586J0619; FLJ46624; integrator complex subunit 1; NET28; UNQ1821/PRO3434 INTS1 INT1, NDCAGF, NET28 integrator complex subunit 1 integrator complex subunit 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on INTS1, check out the INTS1 Infographic
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In this infographic, you will see the following information for INTS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For INTS1 (NM_001080453) Human Over-expression Lysate (LS026173)
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No publications found for LS026173
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