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Product Info Summary
| SKU: | LS009043 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of sperm associated antigen 9 (SPAG9), transcript variant 3 |
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Product info
Product Name
JLP (SPAG9) (NM_003971) Human Over-expression Lysate
SKU/Catalog Number
LS009043
Size
100 µg
Description
Transient overexpression lysate of sperm associated antigen 9 (SPAG9), transcript variant 3
Storage & Handling
Cite This Product
JLP (SPAG9) (NM_003971) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009043)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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JLP (SPAG9) Human Over-expression Lysates NM_003971.
Western blot validation of overexpression lysate (LS009043) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SPAG9 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SPAG9 (Source: Uniprot.org, NCBI)
Gene Name
SPAG9
Full Name
C-Jun-amino-terminal kinase-interacting protein 4
Weight
146.205kDa
Superfamily
JIP scaffold family
Alternative Names
Cancer/testis antigen 89; c-Jun NH2-terminal kinase-associated leucine zipper protein; C-Jun-amino-terminal kinase-interacting protein 4; CT89JIP4; FLJ14006; FLJ26141; FLJ34602; HLC4; HLC-6; HSS; Human lung cancer oncogene 6 protein; JLPPHETSYD1FLJ13450; JNK interacting protein; JNK/SAPK-associated protein; JNK-associated leucine-zipper protein; JNK-interacting protein 4; KIAA0516JIP-4; lung cancer oncogene 4; MAPK8IP4; Max-binding protein; MGC117291; MGC14967; MGC74461; Mitogen-activated protein kinase 8-interacting protein 4; PIG6; proliferation-inducing gene 6; Proliferation-inducing protei SPAG9 CT89, HLC-6, HLC4, HLC6, JIP-4, JIP4, JLP, PHET, PIG6 sperm associated antigen 9 C-Jun-amino-terminal kinase-interacting protein 4|JNK interacting protein|JNK/SAPK-associated protein|Max-binding protein|c-Jun NH2-terminal kinase-associated leucine zipper protein|cancer/testis antigen 89|human lung cancer oncogene 6 protein|lung cancer oncogene 4|mitogen-activated protein kinase 8-interacting protein 4|proliferation-inducing gene 6|protein highly expressed in testis|sperm associated antigen 9 variant 5|sperm surface protein|sunday driver 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SPAG9, check out the SPAG9 Infographic
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In this infographic, you will see the following information for SPAG9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For JLP (SPAG9) (NM_003971) Human Over-expression Lysate (LS009043)
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