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Product Info Summary
| SKU: | LS023210 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of jumonji domain containing 6 (JMJD6), transcript variant 1 |
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Product info
Product Name
JMJD6 (NM_001081461) Human Over-expression Lysate
SKU/Catalog Number
LS023210
Size
100 µg
Description
Transient overexpression lysate of jumonji domain containing 6 (JMJD6), transcript variant 1
Storage & Handling
Cite This Product
JMJD6 (NM_001081461) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS023210)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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JMJD6 Human Over-expression Lysates NM_001081461.
Western blot validation of overexpression lysate (LS023210) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with JMJD6 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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JMJD6 Human Over-expression Lysates NM_015167.
Western blot validation of overexpression lysate (LS023210) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with JMJD6 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for JMJD6 (Source: Uniprot.org, NCBI)
Gene Name
JMJD6
Full Name
Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
Weight
46.462kDa
Superfamily
JMJD6 family
Alternative Names
bifunctional arginine demethylase and lysyl-hydroxylase JMJD6; EC 1.14.11; EC 1.14.11.-; Histone arginine demethylase JMJD6; JmjC domain-containing protein 6; JMJD6; jumonji domain containing 6; Jumonji domain-containing protein 6; KIAA0585PSR; Lysyl-hydroxylase JMJD6; Peptide-lysine 5-dioxygenase JMJD6; Phosphatidylserine receptorPTDSR; Protein PTDSR; PSR; PTDSR; PTDSR1 JMJD6 PSR, PTDSR, PTDSR1 jumonji domain containing 6, arginine demethylase and lysine hydroxylase bifunctional arginine demethylase and lysyl-hydroxylase JMJD6|arginine demethylase and lysine hydroxylase|histone arginine demethylase JMJD6|jmjC domain-containing protein 6|jumonji domain-containing protein 6|lysyl-hydroxylase JMJD6|peptide-lysine 5-dioxygenase JMJD6|phosphatidylserine receptor
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on JMJD6, check out the JMJD6 Infographic
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In this infographic, you will see the following information for JMJD6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For JMJD6 (NM_001081461) Human Over-expression Lysate (LS023210)
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