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Product Info Summary
| SKU: | LS054207 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of potassium channel, subfamily K, member 10 (KCNK10), transcript variant 1 |
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Product info
Product Name
KCNK10 (NM_021161) Human Over-expression Lysate
SKU/Catalog Number
LS054207
Size
100 µg
Description
Transient overexpression lysate of potassium channel, subfamily K, member 10 (KCNK10), transcript variant 1
Storage & Handling
Cite This Product
KCNK10 (NM_021161) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS054207)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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KCNK10 Human Over-expression Lysates NM_021161.
Western blot validation of overexpression lysate (LS054207) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with KCNK10 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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KCNK10 Human Over-expression Lysates NM_138317.
Western blot validation of overexpression lysate (LS054207) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with KCNK10 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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KCNK10 Human Over-expression Lysates NM_138318.
Western blot validation of overexpression lysate (LS054207) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with KCNK10 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for KCNK10 (Source: Uniprot.org, NCBI)
Gene Name
KCNK10
Full Name
Potassium channel subfamily K member 10
Weight
59.765kDa
Superfamily
two pore domain potassium channel (TC 1.A.1.8) family
Alternative Names
2P domain potassium channel TREK2; K2p10.1; Outward rectifying potassium channel protein TREK-2; potassium channel subfamily K member 10; potassium channel TREK-2; potassium channel, subfamily K, member 10; TREK-2 K(+) channel subunit; TREK-2; TREK2FLJ43399; TWIK-related K+ channel 2 KCNK10 K2p10.1, PPP1R97, TREK-2, TREK2 potassium two pore domain channel subfamily K member 10 potassium channel subfamily K member 10|2P domain potassium channel TREK2|TREK-2 K(+) channel subunit|TWIK-related K+ channel 2|outward rectifying potassium channel protein TREK-2|potassium channel TREK-2|potassium channel, subfamily K, member 10|potassium channel, two pore domain subfamily K, member 10|protein phosphatase 1, regulatory subunit 97
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on KCNK10, check out the KCNK10 Infographic
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In this infographic, you will see the following information for KCNK10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For KCNK10 (NM_021161) Human Over-expression Lysate (LS054207)
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No publications found for LS054207
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