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Product Info Summary
| SKU: | LS122042 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of relaxin/insulin-like family peptide receptor 2 (RXFP2), transcript variant 1 |
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Product info
Product Name
LGR8 (RXFP2) (NM_130806) Human Over-expression Lysate
See all Relaxin R2/LGR8 products
SKU/Catalog Number
LS122042
Size
100 µg
Description
Transient overexpression lysate of relaxin/insulin-like family peptide receptor 2 (RXFP2), transcript variant 1
Storage & Handling
Cite This Product
LGR8 (RXFP2) (NM_130806) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS122042)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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LGR8 (RXFP2) Human Over-expression Lysates NM_130806.
Western blot validation of overexpression lysate (LS122042) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RXFP2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for RXFP2 (Source: Uniprot.org, NCBI)
Gene Name
RXFP2
Full Name
Relaxin receptor 2
Weight
86.453kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
G protein coupled receptor affecting testicular descent; Gpr106; GPR106G-protein coupled receptor 106; Great; GREATRelaxin family peptide receptor 2; INSL3R; leucine-rich repeat-containing G protein-coupled receptor 8; Leucine-rich repeat-containing G-protein coupled receptor 8; Lgr8; LGR8.1; LGR8G-protein coupled receptor affecting testicular descent; Relaxin R2; relaxin receptor 2; relaxin/insulin-like family peptide receptor 2; RelaxinR2; RLN2R; Rxfp2; RXFPR2 RXFP2 GPR106, GREAT, INSL3R, LGR8, LGR8.1, RXFPR2 relaxin family peptide receptor 2 relaxin receptor 2|G protein coupled receptor affecting testicular descent|G-protein coupled receptor 106|leucine-rich repeat-containing G-protein coupled receptor 8|relaxin/insulin like family peptide receptor 2
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on RXFP2, check out the RXFP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RXFP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For LGR8 (RXFP2) (NM_130806) Human Over-expression Lysate (LS122042)
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No publications found for LS122042
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