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Product Info Summary
| SKU: | LS006363 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of chemokine (C-C motif) ligand 19 (CCL19) |
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Product info
Product Name
Macrophage Inflammatory Protein 3 beta (CCL19) (NM_006274) Human Over-expression Lysate
See all CCL19/MIP-3 beta products
SKU/Catalog Number
LS006363
Size
100 µg
Description
Transient overexpression lysate of chemokine (C-C motif) ligand 19 (CCL19)
Storage & Handling
Cite This Product
Macrophage Inflammatory Protein 3 beta (CCL19) (NM_006274) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006363)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Macrophage Inflammatory Protein 3 beta (CCL19) Human Over-expression Lysates NM_006274.
Western blot validation of overexpression lysate (LS006363) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CCL19 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CCL19 (Source: Uniprot.org, NCBI)
Gene Name
CCL19
Full Name
C-C motif chemokine 19
Weight
10.993kDa
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19 CCL19 CKb11, ELC, MIP-3b, MIP3B, SCYA19 C-C motif chemokine ligand 19 C-C motif chemokine 19|CC chemokine ligand 19|CK beta-11|EBI1-ligand chemokine|MIP-3-beta|beta chemokine exodus-3|chemokine (C-C motif) ligand 19|epstein-Barr virus-induced molecule 1 ligand chemokine|exodus-3|macrophage inflammatory protein 3-beta|small inducible cytokine subfamily A (Cys-Cys), member 19|small-inducible cytokine A19
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CCL19, check out the CCL19 Infographic
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In this infographic, you will see the following information for CCL19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Macrophage Inflammatory Protein 3 beta (CCL19) (NM_006274) Human Over-expression Lysate (LS006363)
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