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Product Info Summary
| SKU: | LS006490 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of silver homolog (mouse) (SILV) |
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Product info
Product Name
Melanoma gp100 (PMEL) (NM_006928) Human Over-expression Lysate
SKU/Catalog Number
LS006490
Size
100 µg
Description
Transient overexpression lysate of silver homolog (mouse) (SILV)
Storage & Handling
Cite This Product
Melanoma gp100 (PMEL) (NM_006928) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006490)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Melanoma gp100 (PMEL) Human Over-expression Lysates NM_006928.
Western blot validation of overexpression lysate (LS006490) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PMEL (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PMEL (Source: Uniprot.org, NCBI)
Gene Name
PMEL
Full Name
Melanocyte protein PMEL
Weight
70.255kDa
Superfamily
PMEL/NMB family
Alternative Names
D12S53EP1; gp100; ME20; ME20-M; melanocyte protein mel 17; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; melanosomal matrix protein17; PMEL17P100; premelanosome proteinME20M; SI; SIL; silver (mouse homolog) like; silver homolog (mouse); Silver locus protein homolog; silver, mouse, homolog of; SILVPmel17 PMEL D12S53E, ME20, ME20-M, ME20M, P1, P10017, SI, SIL, SILV, gp100, PMEL premelanosome protein melanocyte protein PMEL|melanocyte protein Pmel 17|melanocyte protein mel 17|melanocytes lineage-specific antigen GP100|melanoma-associated ME20 antigen|melanosomal matrix protein17|silver locus protein homolog|silver, mouse, homolog of
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PMEL, check out the PMEL Infographic
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In this infographic, you will see the following information for PMEL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Melanoma gp100 (PMEL) (NM_006928) Human Over-expression Lysate (LS006490)
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No publications found for LS006490
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