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Product Info Summary
| SKU: | EK0566 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse CCL1/I 309 ELISA Kit PicoKine®
View all CCL1/I-309/TCA-3 ELISA kits
SKU/Catalog Number
EK0566
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CCL1/I 309 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccl1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CCL1/I 309 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0566)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: K24-C92
Sensitivity
<1 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0566 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0566 is reactive to CCL1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0566 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL1/I-309/TCA-3
CCL1, Chemokine (C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0566-CAP | Anti-Mouse CCL1 Pre-coated 96-well strip microplate | 1 |
| EK0566-ST | Mouse CCL1 Standard | 2 vials, 10 ng/tube |
| EK0566-DA | Mouse CCL1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.029 | 0.098 | 0.157 | 0.318 | 0.574 | 1.144 | 1.630 | 2.067 |
Data Example Images
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Mouse CCL1/TCA3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CCL1/I 309 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 17 | 81 | 291 | 16 | 78 | 277 |
| Standard deviation | 0.9 | 5.5 | 18.91 | 1.02 | 6.78 | 20.22 |
| CV (%) | 5.3% | 6.8% | 6.5% | 6.4% | 8.7% | 7.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL1/I-309/TCA-3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 17 | 15 | 16 | 16 | 16 | 0.7 | 4.3% |
| Sample 2 | 81 | 79 | 85 | 79 | 81 | 2.44 | 3% |
| Sample 3 | 291 | 314 | 273 | 296 | 293 | 14.6 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For CCL1 (Source: Uniprot.Org, NCBI)
Gene Name
CCL1
Full Name
C-C motif chemokine 1
Weight
10.992kDa
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3 CCL1 I-309, P500, SCYA1, SISe, TCA3 C-C motif chemokine ligand 1 C-C motif chemokine 1|T lymphocyte-secreted protein I-309|chemokine (C-C motif) ligand 1|inflammatory cytokine I-309|small inducible cytokine A1 (I-309, homologous to mouse Tca-3)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCL1, check out the CCL1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CCL1/I 309 ELISA Kit PicoKine® (EK0566)
Hello CJ!
EK0566 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Contribution of PPARγ in modulation of acrolein-induced inflammatory signaling in gp91phox knock-out mice
Blockage of C-C Chemokine Ligand 2 Alleviated the Appendicitis-Induced Injury in Rabbit Model
A “sandwich” cell culture platform with NIR-responsive dynamic stiffness to modulate macrophage phenotypes
Near-Infrared-Triggered Dynamic Surface Topography for Sequential Modulation of Macrophage Phenotypes
Systemic delivery of TNF-armed myxoma virus plus immune checkpoint inhibitor eliminates lung metastatic mouse osteosarcoma
Extracted Triterpenes from Antrodia cinnamomea Reduce the Inflammation to Promote the Wound Healing via the STZ Inducing Hyperglycemia-Diabetes Mice Model
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
6 Customer Q&As for Mouse CCL1/I 309 ELISA Kit PicoKine®
Question
Q: how to analyze ELISA data? I have performed CCL1 level in serum.
M. Zhao
Verified customer
Asked: 2019-02-10
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-02-10
Question
Q: Can CCL1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
M. Dhawan
Verified customer
Asked: 2018-06-26
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-06-26
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-04-02
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-04-02
Question
Q: if the enzyme conjugated CCL1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL1 antigen?
J. Scott
Verified customer
Asked: 2017-07-04
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-07-04
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2017-01-18
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-01-18
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Green
Verified customer
Asked: 2014-06-05
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2014-06-05
