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Product Info Summary
| SKU: | EK1458 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. |
Product info
Product Name
Mouse FABP4/A Fabp ELISA Kit PicoKine®
View all FABP4/A-FABP ELISA kits
SKU/Catalog Number
EK1458
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse FABP4/A Fabp ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Fabp4 in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse FABP4/A Fabp ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1458)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: M1-A132
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1458 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1458 is reactive to FABP4 in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.
Application Guarantee
EK1458 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FABP4/A-FABP
FABP4, also known as aP2 (adipocyte Protein 2), encodes the fatty acid binding protein found in adipocytes. The FABP4 gene is mapped to 8q21. FABP4 functions as a cytosolic lipid chaperone in macrophages and is involved in regulating macrophage endoplasmic reticulum (ER) stress. Aditionally, FABP4 protein is expressed in HBEs and is strongly upregulated by both IL4 and IL13 and downregulated by IFN-gamma, suggesting a role in allergic airway inflammation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1458-CAP | Anti-Mouse FABP4 Pre-coated 96-well strip microplate | 1 |
| EK1458-ST | Mouse FABP4 Standard | 2 vials, 10 ng/tube |
| EK1458-DA | Mouse FABP4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.123 | 0.257 | 0.393 | 0.597 | 1.027 | 1.478 | 1.816 | 2.232 |
Data Example Images
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Mouse FABP4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FABP4/A-FABP, Dilution ratio of 1:50, concentration in serum and plasma is 20-100 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse FABP4/A Fabp ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 344 | 1350 | 4564 | 359 | 1418 | 4994 |
| Standard deviation | 26.83 | 74.25 | 351.42 | 29.07 | 93.58 | 409.5 |
| CV (%) | 7.8% | 5.5% | 7.7% | 8.1% | 6.6% | 8.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FABP4/A-FABP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 344 | 313 | 309 | 323 | 322 | 13.55 | 4.2% |
| Sample 2 | 1350 | 1278 | 1457 | 1510 | 1398 | 90.45 | 6.4% |
| Sample 3 | 4564 | 4763 | 4358 | 4101 | 4446 | 245.55 | 5.5% |
Protein Target Info & Infographic
Gene/Protein Information For FABP4 (Source: Uniprot.Org, NCBI)
Gene Name
FABP4
Full Name
Fatty acid-binding protein, adipocyte
Weight
14.719kDa
Superfamily
calycin superfamily
Alternative Names
Adipocyte lipid-binding protein; Adipocyte-type fatty acid-binding protein; AFABP; A-FABP; A-FABPAFABP; ALBP; aP2; FABP4; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; fatty acid-binding protein, adipocyte FABP4 A-FABP, AFABP, ALBP, HEL-S-104, aP2 fatty acid binding protein 4 fatty acid-binding protein, adipocyte|adipocyte fatty acid binding protein|adipocyte lipid-binding protein|adipocyte-type fatty acid-binding protein|epididymis secretory protein Li 104|fatty acid binding protein 4, adipocyte
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FABP4, check out the FABP4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FABP4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse FABP4/A Fabp ELISA Kit PicoKine® (EK1458)
Hello CJ!
EK1458 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Altered fatty acid‐binding protein 4 (FABP4) expression and function in human and animal models of hepatocellular carcinoma
FABP4 contributes to renal interstitial fibrosis via mediating inflammation and lipid metabolism
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Customer Reviews
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1 Reviews For Mouse FABP4/A Fabp ELISA Kit PicoKine®
0
No Complaints. A Simple, Effective ELISA from BosterBio--David Nelson, University of Wisconsin, Research Scientist
Excellent
Source: Biocompare.com
| Application | ELISA |
|---|---|
| Sample | Mouse plasma (with EDTA as anticoagulant) |
| Detection | -- |
"Reagents are easy to prep or already 1X. Two vials of standard allows for breaking the kit into two assays. 10 ml of TMB and Stop solution is cutting it close, but was not an issue. Really nothing was bad. Standard curve was easy to make, and 4-parameter curve fit perfect (R2>0.99). CV% for replicates on standard curve were a bit higher than I expected - around 5-10% - but consistent with the product literature. I'd recommend the kit. I did the assay at room temperature (instead of the recommended 37°C) because I didn't want to deal with going back and forth into the room that houses our 37°C incubator. Doing the assay at room temperature takes a bit longer (for the incubations). The data looked good (based on R2 for standard curve and CV% for replicates. In the image, Columns A and B are the standard curve." (Customer’s Tip: "100-fold dilution of mouse plasma resulted in all data falling on standard curve.")
David Nelson
Verified customer
Submitted 2019-12-06
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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Submit your question
9 Customer Q&As for Mouse FABP4/A Fabp ELISA Kit PicoKine®
Question
Q: Can FABP4 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-07-23
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-07-23
Question
Q: how do I analyze ELISA data? I have obtained FABP4 level in plasma.
T. Tiwari
Verified customer
Asked: 2020-07-11
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-07-11
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-03-11
Question
Q: can you suggest the dilution ratio of serum samples for detection of FABP4 in Mouse plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
H. Rodriguez
Verified customer
Asked: 2020-03-07
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the FABP4 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-03-07
Question
What is the suggested dilution ratio for mouse serum samples for EK1458?
Verified customer
Asked: 2019-08-26
Answer
For the Mouse FABP4/A Fabp ELISA Kit PicoKine™ (EK1458), the suggested dilution for mouse serum samples is 1:100.
Boster Scientific Support
Answered: 2019-08-27
Question
Q: if the enzyme conjugated FABP4 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FABP4 antigen?
Verified Customer
Verified customer
Asked: 2018-12-13
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-12-13
Question
Q: can I use citrate plasma as samples in Mouse FABP4 Picokine® ELISA Kit (Catalog # EK1458)?
P. Zhao
Verified customer
Asked: 2018-06-07
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of FABP4 causing disruption of its protein structure. FABP4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the FABP4 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-06-07
Question
Has EK1458 been tested for cross-reactivity with human samples?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
EK1458 has been tested for cross-reactivity with human samples. No significant cross-reactivity or interference was observed with human.
Boster Scientific Support
Answered: 2017-10-25
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement FABP4 using the EK1458 Mouse FABP4 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2015-02-10
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2015-02-10
