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Product Info Summary
| SKU: | EK2073 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse GFRA2 ELISA Kit PicoKine®
View all GFR alpha-2/GDNF R alpha-2 ELISA kits
SKU/Catalog Number
EK2073
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse GFRA2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Gfra2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse GFRA2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2073)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S22-S443
Sensitivity
<50 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2073 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2073 is reactive to GFRA2 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2073 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GFR alpha-2/GDNF R alpha-2
GDNF family receptor alpha-2 (GFRα2), also known as the neurturin receptor, is a protein that in humans is encoded by the GFRA2 gene. It is mapped to 14; 14 D2. The GFRA2 protein is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor. It is part of the GDNF receptor family. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. They both bind the GFRA2 receptor. The receptor mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. GFRA2 (gene) has been shown to interact with GDNF.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2073-CAP | Anti-Mouse GFRA2 Pre-coated 96-well strip microplate | 1 |
| EK2073-ST | Mouse GFRA2 Standard | 2 vials, 20 ng/tube |
| EK2073-DA | Mouse GFRA2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.072 | 0.117 | 0.199 | 0.249 | 0.678 | 1.090 | 1.434 | 2.227 |
Data Example Images
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Mouse GFRA2 PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse GFRA2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 536 | 2108 | 8768 | 582 | 2224 | 9685 |
| Standard deviation | 23.05 | 94.86 | 412.10 | 32.59 | 128.99 | 600.47 |
| CV (%) | 4.3% | 4.5% | 4.7% | 5.6% | 5.8% | 6.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of GFR alpha-2/GDNF R alpha-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 536 | 499 | 569 | 494 | 524 | 30.38 | 5.7% |
| Sample 2 | 2108 | 2258 | 2305 | 2207 | 2219 | 73.11 | 3.2% |
| Sample 3 | 8768 | 8608 | 9463 | 9664 | 9125 | 447.07 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For GFRA2 (Source: Uniprot.Org, NCBI)
Gene Name
GFRA2
Full Name
GDNF family receptor alpha-2
Weight
51.544kDa
Superfamily
GDNFR family
Alternative Names
GDNF family receptor alpha 2; GDNF family receptor alpha-2; GDNF R alpha-2; GDNF receptor alpha-2; GDNF receptor beta; GDNFR-alpha-2; GDNFR-beta; GDNFRBRET ligand 2; GFR alpha2; GFR alpha-2; GFRA2; GFRa-2; GFR-alpha 2; GFR-alpha-2; glial cell line derived neurotrophic factor receptor, beta; Neurturin receptor alpha; NRTNR-alpha; NTNRA; NTNR-alpha; PI-linked cell-surface accessory protein; RETL2TGF-beta-related neurotrophic factor receptor 2; TGF-beta related neurotrophic factor receptor 2; TRN receptor, GPI-anchored; TRNR2NRTNR-ALPHA GFRA2 GDNFRB, NRTNR-ALPHA, NTNRA, RETL2, TRNR2 GDNF family receptor alpha 2 GDNF family receptor alpha-2|GDNF receptor beta|GDNFR-alpha-2|GDNFR-beta|GFR-alpha 2|NTNR-alpha|PI-linked cell-surface accessory protein|RET ligand 2|TGF-beta-related neurotrophic factor receptor 2|TRN receptor, GPI-anchored|glial cell line derived neurotrophic factor receptor, beta|neurturin receptor alpha
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GFRA2, check out the GFRA2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GFRA2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse GFRA2 ELISA Kit PicoKine® (EK2073)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Mouse GFRA2 ELISA Kit PicoKine®
Question
Q: how to thaw whole blood sample for GFR alpha-2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test GFR alpha-2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-01-13
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of GFR alpha-2 in Mouse plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the GFR alpha-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-12-27
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-05-24
Question
Q: can I use heparin plasma as samples in Mouse GFR alpha-2 Picokine® ELISA Kit (Catalog # EK2073)?
D. Sunder
Verified customer
Asked: 2018-08-24
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of GFR alpha-2 causing degradation of its protein structure. GFR alpha-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the GFR alpha-2 ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-08-24
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring GFR alpha-2 using the EK2073 Mouse GFR alpha-2 Picokine® ELISA Kit?
A. Puri
Verified customer
Asked: 2018-08-16
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-08-16
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
K. Singh
Verified customer
Asked: 2018-02-26
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-02-26
Question
Q: how to analyze ELISA data? I have obtained GFR alpha-2 level in plasma.
Verified Customer
Verified customer
Asked: 2017-11-16
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-11-16
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
M. Edwards
Verified customer
Asked: 2014-02-25
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2014-02-25
