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Product Info Summary
| SKU: | EK1453 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine®
View all Iduronate 2-Sulfatase/IDS ELISA kits
SKU/Catalog Number
EK1453
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ids in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1453)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: T36-P552
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1453 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1453 is reactive to IDS in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1453 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Iduronate 2-Sulfatase/IDS
Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1453-CAP | Anti-Mouse IDS Pre-coated 96-well strip microplate | 1 |
| EK1453-ST | Mouse IDS Standard | 2 vials, 10 ng/tube |
| EK1453-DA | Mouse IDS Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.070 | 0.196 | 0.268 | 0.473 | 0.634 | 1.083 | 1.633 | 2.299 |
Data Example Images
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Mouse IDS PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Iduronate 2-Sulfatase/IDS, Dilution ratio of 1:5, concentration in serum and plasma is around 5.5-7.5 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 292 | 1534 | 5259 | 287 | 1421 | 5751 |
| Standard deviation | 13.14 | 108.91 | 241.91 | 16.07 | 102.31 | 317.2% |
| CV (%) | 4.5% | 7.1% | 4.6% | 5.6% | 7.2% | 5.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Iduronate 2-Sulfatase/IDS in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 292 | 332 | 309 | 275 | 302 | 21.08 | 6.9% |
| Sample 2 | 1534 | 1618 | 1672 | 1683 | 1626 | 58.92 | 3.6% |
| Sample 3 | 5259 | 5568 | 5740 | 5102 | 5417 | 250.65 | 4.6% |
Protein Target Info & Infographic
Gene/Protein Information For IDS (Source: Uniprot.Org, NCBI)
Gene Name
IDS
Full Name
Iduronate 2-sulfatase
Weight
61.873kDa
Superfamily
sulfatase family
Alternative Names
Alpha-L-iduronate sulfate sulfatase; EC 3.1.6.13; IDS; iduronate 2-sulfatase 14 kDa chain; iduronate 2-sulfatase 42 kDa chain; iduronate 2-sulfatase; idursulfase; MPS2; S; SIDS IDS ID2S, MPS2, SIDS iduronate 2-sulfatase iduronate 2-sulfatase|alpha-L-iduronate sulfate sulfatase|iduronate 2-sulfatase 14 kDa chain|iduronate 2-sulfatase 42 kDa chain|idursulfase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IDS, check out the IDS Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IDS: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine® (EK1453)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Mouse IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
B. Varadkar
Verified customer
Asked: 2020-05-09
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-05-09
Question
Q: What is the optimal O.D. value for IDS ELISA kit? I used your IDS ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IDS even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IDS ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of IDS that can be declared/interpreted as positive by the above standard is 15pg/ml.
Boster Scientific Support
Answered: 2018-10-05
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of IDS in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
B. Dhar
Verified customer
Asked: 2018-08-25
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IDS ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-08-25
Question
Q: if the enzyme conjugated IDS antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IDS antigen?
L. Roberts
Verified customer
Asked: 2018-06-26
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-06-26
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-10-31
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
K. Chakrabarti
Verified customer
Asked: 2016-05-15
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-05-15
Question
Q: is it okay to use citrate plasma as samples in Mouse IDS Picokine® ELISA Kit (Catalog # EK1453)?
Verified Customer
Verified customer
Asked: 2016-02-27
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of IDS causing disruption of its protein structure. IDS may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the IDS ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-02-27
