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Product Info Summary
| SKU: | A00180 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Iduronate 2-sulfatase IDS Antibody
SKU/Catalog Number
A00180
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Iduronate 2-sulfatase IDS Antibody catalog # A00180. Tested in WB,IHC,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Iduronate 2-sulfatase IDS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00180)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human IDS(NP_006114.1).
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00180 is reactive to IDS in Human, Mouse, Rat
Calculated molecular weight
61.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00180 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using IDS antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat brain using IDS Rabbit pAb at dilution of 1:200 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Immunohistochemistry of paraffin-embedded mouse testis using IDS Rabbit pAb at dilution of 1:200 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Immunofluorescence analysis of NIH/3T3 cells using IDS Rabbit pAb at dilution of 1:25 . Blue: DAPI for nuclear staining.
Specific Publications For Anti-Iduronate 2-sulfatase IDS Antibody (A00180)
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