Mouse Lipocalin-2/NGAL ELISA Kit PicoKine™

Mouse Lipocalin-2/NGAL ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Lcn2 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. Cited in 5 publication(s).

Product Info Summary

SKU: EK0854
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Mouse Lipocalin-2/NGAL ELISA Kit PicoKine™

See all Lipocalin-2/NGAL products

SKU/Catalog Number

EK0854

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Lipocalin-2/NGAL ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Lcn2 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse Lipocalin-2/NGAL ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0854)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-N200

Sensitivity

<10 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0854 is reactive to Lcn2 in Mouse samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK0854 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0854-CAP Anti-Mouse Lcn2 Pre-coated 96-well strip microplate 1
EK0854-ST Mouse Lcn2 Standard 2 vials, 10 ng/tube
EK0854-DA Mouse Lcn2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156312625125025005000
O.D.0.0080.1030.1750.3160.6240.9921.6002.067

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Lipocalin-2/NGAL, Dilution ratio of 1:500, concentration in serum is 160-800ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Lipocalin-2/NGAL ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)14178525881308172606
Standard deviation6.238.46113.877.5454.73161.57
CV (%)4.4%4.9%4.4%5.8%6.7%6.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Lipocalin-2/NGAL in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11411321301421365.33.8%
Sample 278577674683978633.574.2%
Sample 325882673221324412478174.437%
*number of samples for each test n=16.

Gene/Protein Information For Lcn2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P11672

Gene ID

16819

Gene Name

Lcn2

Full Name

Neutrophil gelatinase-associated lipocalin

Weight

22.875kDa

Superfamily

calycin superfamily

Alternative Names

24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin Lcn2|24p, 24p3, AW212229, N, NRL, Sip24|lipocalin 2|neutrophil gelatinase-associated lipocalin|SV-40-induced 24P3 protein|neu-related lipocalin|oncogene 24p3|p25|secreted inducible protein 24|siderocalin LCN2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Lcn2, check out the Lcn2 Infographic

Lcn2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Lcn2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0854 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li H,Sun H,Xu Y,Xing G,Wang X.Curcumin plays a protective role against septic acute kidney injury by regulating the TLR9 signaling pathway.Transl Androl Urol.2021 May;10(5):2103-2112.doi:10.21037/tau-21-385.PMID:34159091;PMCID:PMC8185681.
Species: Rat
EK0854 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Koca U, Olguner ??g, Erg??r Bu, Altekin E, Ta??d????en A, Duru S, Girgin P, G??nd??z K, Cilaker M??c??l?? S, G??zelda?? S, Akku?? M. Scientificworldjournal. 2013;2013:292687. Doi: 10.1155/2013/292687. Epub 2013 Feb 13. The Effects Of Dexmedetomidi...

Olguner Cg, Koca U, Altekin E, Erg??r Bu, Duru S, Girgin P, Ta??d????en A, G??nd??z K, G??zelda?? S, Akku?? M, Micili Sc. Exp Ther Med. 2013 Jun;5(6):1581-1588. Epub 2013 Apr 2. Ischemic Preconditioning Attenuates Lipid Peroxidation And Apoptosis ...

Sun Q, Meng Qt, Jiang Y, Liu Hm, Lei Sq, Su Wt, Duan Wn, Wu Y, Xia Zy, Xia Zy. Plos One. 2013 Dec 4;8(12):E80859. Doi: 10.1371/Journal.Pone.0080859. Ecollection 2013. Protective Effect Of Ginsenoside Rb1 Against Intestinal Ischemia-Reperfusion Ind...

Liu W, Dong Xs, Sun Yq, Liu Z. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. Ecollection 2014. A Novel Fluid Resuscitation Protocol: Provide More Protection On Acute Kidney Injury During Septic Shock In Rats.

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Have a question?

Find answers in Q&As, reviews.

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8 Customer Q&As for Mouse Lipocalin-2/NGAL ELISA Kit PicoKine™

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

J. Baker

Verified customer

Asked: 2020-09-13

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-09-13

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

D. Campbell

Verified customer

Asked: 2020-08-22

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-08-22

Question

Q: if the enzyme conjugated Lipocalin-2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Lipocalin-2 antigen?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-12-25

Question

Is the Mouse Lipocalin-2/NGAL ELISA Kit PicoKine™ (EK0854) suitable for samples of plasma with EDTA?

Verified customer

Asked: 2019-12-06

Answer

Yes, the Mouse Lipocalin-2/NGAL ELISA Kit PicoKine™ (EK0854) is suitable for samples of plasma with EDTA.

Boster Scientific Support

Answered: 2019-12-06

Question

Q: how to analyze ELISA data? I have obtained Lipocalin-2 level in urine.

Verified Customer

Verified customer

Asked: 2019-05-07

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-05-07

Question

Q: Can Lipocalin-2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2018-08-19

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-08-19

Question

Q: What is the optimal O.D. value for Lipocalin-2 ELISA kit? I performed your Lipocalin-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Lipocalin-2 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-03-25

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Lipocalin-2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Lipocalin-2 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-03-25

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2016-03-01

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2016-03-01

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