Mouse Progranulin PicoKine® Quick ELISA Kit

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Mouse Progranulin PicoKine® Quick ELISA Kit

See all GRN products

SKU/Catalog Number

FEK1192

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Progranulin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Grn in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse Progranulin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1192)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: T18-L589

Sensitivity

<10 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Standard Dilution Instructions

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1192 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1192 is reactive to GRN in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK1192 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of GRN

Granulin, also known as Proepithelin (PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Kit Components

Catalog number	Description	Quantity
FEK1192-CAP	96-well plate precoated with anti-Mouse GRN antibody	1
EK1192-ST	lyophilized recombinant Mouse GRN standard	50 ng/tube
FEK1192-DA	Biotinylated anti-Mouse GRN detection antibody (50x)	100 ul
-	Avidin-Biotin-Peroxidase Complex (30x)	400 µl
AR1106-1	Sample diluent	30 ml
AR1106-2	Antibody diluent	12 ml
-	Avidin-Biotin-Peroxidase Diluent	12 ml
AR1106-5	Wash Buffer (25x)	20 ml
AR1104	Color Developing Reagent (TMB)	10 ml
AR1105	Stop Solution	10 ml
PLA-SEA	Plate Sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.001	0.175	0.344	0.550	1.025	1.496	1.816	2.000

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of GRN in ELISA kits from four different production batches/lots.

Gene/Protein Information For GRN (Source: Uniprot.Org, NCBI)

Alternative Names

Acrogranin; CLN11; GEP; GP88; granulin precursor; GRN; PCDGF; PEPI; PGRN; Proepithelin; Progranulin GRN CLN11, GEP, GP88, PCDGF, PEPI, PGRN granulin precursor progranulin|PC cell-derived growth factor|acrogranin|epithelin|glycoprotein 88|glycoprotein of 88 Kda|granulin-epithelin|granulins|proepithelin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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