Mouse Progranulin ELISA Kit PicoKine™

Mouse Progranulin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Grn in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1192
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse Progranulin ELISA Kit PicoKine™

See all Grn products

SKU/Catalog Number

EK1192

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Progranulin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Grn in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse Progranulin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1192)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: T18-L589

Sensitivity

<10 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1192 is reactive to Grn in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1192 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1192-CAP Anti-Mouse Grn Pre-coated 96-well strip microplate 1
EK1192-ST Mouse Grn Standard 2 vials, 50 ng/tube
EK1192-DA Mouse Grn Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)00.781.563.126.2512.52550
O.D.0.0010.1750.3440.5501.0251.4961.8162.000

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Progranulin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)20077073239341896699222153
Standard deviation130.45339.51006.5%2144.09349.61019.03
CV (%)6.5%4.8%4.2%7.6%5%4.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Grn in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12007210618881899197588.784.4%
Sample 270737342731169757175155.552.1%
Sample 32393424448247582423324343301.091.2%
*number of samples for each test n=16.

Gene/Protein Information For Grn (Source: Uniprot.Org, NCBI)

Uniprot ID

P28798

Gene ID

14824

Gene Name

Grn

Full Name

Progranulin

Weight

63.458kDa

Superfamily

granulin family

Alternative Names

Acrogranin; CLN11; GEP; GP88; granulin precursor; GRN; PCDGF; PEPI; PGRN; Proepithelin; Progranulin Grn|GP88, P, PCDGF, PEPI, Pgrn, epit, epithelin, pr|granulin|progranulin|PC cell-derived growth factor|acrogranin|acrogranulin|epithelin/granulin|glycoprotein 88|glycoprotein of 88 Kda|granulins|proepithelin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Grn, check out the Grn Infographic

Grn infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Grn: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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9 Customer Q&As for Mouse Progranulin ELISA Kit PicoKine™

Question

Q: What is the optimal O.D. value for GRN ELISA kit? I used your GRN ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain GRN even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-12-17

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this GRN ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of GRN that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2020-12-17

Question

Q: is it okay to use citrate plasma as samples in Mouse GRN Picokine® ELISA Kit (Catalog # EK1192)?

Z. Green

Verified customer

Asked: 2020-07-02

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of GRN causing degradation of its protein structure. GRN may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the GRN ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-07-02

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

M. Anand

Verified customer

Asked: 2020-03-11

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-03-11

Question

Q: how to proceed with the analysis of ELISA data? I have performed GRN level in serum.

Verified Customer

Verified customer

Asked: 2019-08-21

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-08-21

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-10-25

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-10-25

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-07-28

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-07-28

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-03-20

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-03-20

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification GRN using the EK1192 Mouse GRN Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-11-03

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2017-11-03

Question

Q: if the enzyme conjugated GRN antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the GRN antigen?

Verified Customer

Verified customer

Asked: 2014-03-09

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2014-03-09

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