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Product Info Summary
| SKU: | LS009252 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of ribosomal protein S6 kinase, 90kDa, polypeptide 5 (RPS6KA5), transcript variant 1 |
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Product info
Product Name
MSK1 (RPS6KA5) (NM_004755) Human Over-expression Lysate
SKU/Catalog Number
LS009252
Size
100 µg
Description
Transient overexpression lysate of ribosomal protein S6 kinase, 90kDa, polypeptide 5 (RPS6KA5), transcript variant 1
Storage & Handling
Cite This Product
MSK1 (RPS6KA5) (NM_004755) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009252)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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MSK1 (RPS6KA5) Human Over-expression Lysates NM_004755.
Western blot validation of overexpression lysate (LS009252) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RPS6KA5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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MSK1 (RPS6KA5) Human Over-expression Lysates NM_182398.
Western blot validation of overexpression lysate (LS009252) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RPS6KA5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for RPS6KA5 (Source: Uniprot.org, NCBI)
Gene Name
RPS6KA5
Full Name
Ribosomal protein S6 kinase alpha-5
Weight
89.865kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; MGC1911,90 kDa ribosomal protein S6 kinase 5; MSK1; MSK1EC 2.7.11.1; MSPK1; Nuclear mitogen- and stress-activated protein kinase 1; ribosomal protein S6 kinase alpha-5; ribosomal protein S6 kinase, 90kD, polypeptide 5; ribosomal protein S6 kinase, 90kDa, polypeptide 5; RLPK; RPS6KA5; RSKL; RSK-like protein kinase; S6K-alpha-5 RPS6KA5 MSK1, MSPK1, RLPK ribosomal protein S6 kinase A5 ribosomal protein S6 kinase alpha-5|90 kDa ribosomal protein S6 kinase 5|RSK-like protein kinase|RSKL|S6K-alpha-5|nuclear mitogen- and stress-activated protein kinase 1|ribosomal protein S6 kinase, 90kDa, polypeptide 5
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on RPS6KA5, check out the RPS6KA5 Infographic
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In this infographic, you will see the following information for RPS6KA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For MSK1 (RPS6KA5) (NM_004755) Human Over-expression Lysate (LS009252)
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