Click image to see more details
Product Info Summary
| SKU: | LS005442 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of polymerase (RNA) mitochondrial (DNA directed) (POLRMT), nuclear gene encoding mitochondrial protein |
Customers Who Bought This Also Bought
Product info
Product Name
mtRNA polymerase (POLRMT) (NM_005035) Human Over-expression Lysate
See all mtRNA polymerase products
SKU/Catalog Number
LS005442
Size
100 µg
Description
Transient overexpression lysate of polymerase (RNA) mitochondrial (DNA directed) (POLRMT), nuclear gene encoding mitochondrial protein
Storage & Handling
Cite This Product
mtRNA polymerase (POLRMT) (NM_005035) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005442)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
mtRNA polymerase (POLRMT) Human Over-expression Lysates NM_005035.
Western blot validation of overexpression lysate (LS005442) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with POLRMT (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for POLRMT (Source: Uniprot.org, NCBI)
Gene Name
POLRMT
Full Name
DNA-directed RNA polymerase, mitochondrial
Weight
138.62kDa
Superfamily
phage and mitochondrial RNA polymerase family
Alternative Names
APOLMTEC 2.7.7.6; DNA-directed RNA polymerase, mitochondrial; h-mtRPOL; MTRNAP; MTRPOL; polymerase (RNA) mitochondrial (DNA directed) POLRMT APOLMT, MTRNAP, MTRPOL, h-mtRPOL RNA polymerase mitochondrial DNA-directed RNA polymerase, mitochondrial|polymerase (RNA) mitochondrial (DNA directed)
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on POLRMT, check out the POLRMT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLRMT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For mtRNA polymerase (POLRMT) (NM_005035) Human Over-expression Lysate (LS005442)
Hello CJ!
No publications found for LS005442
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful:
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used mtRNA polymerase (POLRMT) (NM_005035) Human Over-expression Lysate?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review mtRNA polymerase (POLRMT) (NM_005035) Human Over-expression Lysate
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
