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Product Info Summary
| SKU: | LS022861 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of NLR family, pyrin domain containing 1 (NLRP1), transcript variant 1 |
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Product info
Product Name
NALP1 (NLRP1) (NM_033004) Human Over-expression Lysate
SKU/Catalog Number
LS022861
Size
100 µg
Description
Transient overexpression lysate of NLR family, pyrin domain containing 1 (NLRP1), transcript variant 1
Storage & Handling
Cite This Product
NALP1 (NLRP1) (NM_033004) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS022861)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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NALP1 (NLRP1) Human Over-expression Lysates NM_033004.
Western blot validation of overexpression lysate (LS022861) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with NLRP1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for NLRP1 (Source: Uniprot.org, NCBI)
Gene Name
NLRP1
Full Name
NACHT, LRR and PYD domains-containing protein 1
Weight
165.866kDa
Superfamily
NLRP family
Alternative Names
CARD7; CARD7SLEV1; Caspase recruitment domain-containing protein 7; CLR17.1; Death effector filament-forming ced-4-like apoptosis protein; DEFCAP; DEFCAPVAMAS1; DKFZp586O1822; KIAA0926DEFCAP-L/S; NACHT, leucine rich repeat and PYD (pyrin domain) containing 1; NACHT, leucine rich repeat and PYD containing 1; NACHT, LRR and PYD containing protein 1; NACHT, LRR and PYD domains-containing protein 1; NACPP1044; NALP1; NALP1caspase recruitment domain protein 7; NLR family, pyrin domain containing 1; NLRP1; Nucleotide-binding domain and caspase recruitment domain; nucleotide-binding oligomerization d NLRP1 AIADK, CARD7, CIDED, CLR17.1, DEFCAP, DEFCAP-L/S, JRRP, MSPC, NAC, NALP1, PP1044, SLEV1, VAMAS1 NLR family pyrin domain containing 1 NACHT, LRR and PYD domains-containing protein 1|NACHT, LRR and PYD containing protein 1|NACHT, leucine rich repeat and PYD (pyrin domain) containing 1|NACHT, leucine rich repeat and PYD containing 1|caspase recruitment domain protein 7|caspase recruitment domain-containing protein 7|death effector filament-forming Ced-4-like apoptosis protein|nucleotide-binding domain and caspase recruitment domain|nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on NLRP1, check out the NLRP1 Infographic
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In this infographic, you will see the following information for NLRP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For NALP1 (NLRP1) (NM_033004) Human Over-expression Lysate (LS022861)
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