Anti-NALP1 NLRP1 Antibody

Product Name

Anti-NALP1 NLRP1 Antibody

View all NLRP1 Antibodies

SKU/Catalog Number

A00777

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NALP1 NLRP1 Antibody (Catalog # A00777). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.

Storage & Handling

NALP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-NALP1 NLRP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00777)

Host

Rabbit

Contents

NALP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

NALP1 antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human NALP1. The immunogen is located within the last 50 amino acids of NALP1.

Reactive Species

A00777 is reactive to NLRP1 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

165.9 kDa

Background of NLRP1

NALP1 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli. It contains a caspase recruitment domain (CARD) in addition to a NACHT domain and leucine rich repeat (LRR). This protein interacts strongly with caspase 2 and weakly with caspase 9, as well as with Apaf-1. Transient overexpression of this gene in cultured cells was sufficient to induce apoptosis. NALP1 mRNA and protein expression levels in myeloid leukemia cells are increased following CREB (cAMP-response element binding protein) activation, suggesting that NALP1 may contribute to modulate the response of these cells to pro-inflammatory stimuli. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for NALP1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00777 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NALP1 antibody can be used for detection of NALP1 by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information NLRP1

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