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Product Info Summary
| SKU: | LS064067 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of neuronal PAS domain protein 3 (NPAS3), transcript variant 2 |
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Product info
Product Name
NPAS3 (NM_022123) Human Over-expression Lysate
SKU/Catalog Number
LS064067
Size
100 µg
Description
Transient overexpression lysate of neuronal PAS domain protein 3 (NPAS3), transcript variant 2
Storage & Handling
Cite This Product
NPAS3 (NM_022123) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS064067)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Protein Target Info & Infographic
Gene/Protein Information for Npas3 (Source: Uniprot.org, NCBI)
Gene Name
Npas3
Full Name
Neuronal PAS domain-containing protein 3
Weight
100.458kDa
Alternative Names
Basic-helix-loop-helix-PAS protein MOP6; BHLHE12; bHLHe12FLJ11605; Class E basic helix-loop-helix protein 12; Member of PAS protein 6; MOP6FLJ10003; neuronal PAS domain protein 3; neuronal PAS domain-containing protein 3; Neuronal PAS3; PAS domain-containing protein 6; PASD6FLJ11138 Npas3|4930423H22Rik, bHLHe1, bHLHe12|neuronal PAS domain protein 3|neuronal PAS domain-containing protein 3|basic-helix-loop-helix-PAS protein MOP6|member of PAS protein 6|neuronal PAS3
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Npas3, check out the Npas3 Infographic
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In this infographic, you will see the following information for Npas3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For NPAS3 (NM_022123) Human Over-expression Lysate (LS064067)
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