NTH1 (NTHL1) (NM_002528) Human Over-expression Lysate

Lysate of NTH1 over-expression cell lines, WB control

Transient overexpression lysate of nth endonuclease III-like 1 (E. coli) (NTHL1)

Product Info Summary

SKU: LS004913
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of nth endonuclease III-like 1 (E. coli) (NTHL1)

Product Name

NTH1 (NTHL1) (NM_002528) Human Over-expression Lysate

See all NTH1 products

SKU/Catalog Number

LS004913

Size

100 µg

Description

Transient overexpression lysate of nth endonuclease III-like 1 (E. coli) (NTHL1)

Storage & Handling

Cite This Product

NTH1 (NTHL1) (NM_002528) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS004913)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for NTHL1 (Source: Uniprot.org, NCBI)

Gene Name

NTHL1

Full Name

Endonuclease III-like protein 1

Weight

34.39kDa

Superfamily

Nth/MutY family

Alternative Names

EC 4.2.99.18; endonuclease III-like protein 1; nth endonuclease III-like 1 (E. coli); NTH1; NTHL1; OCTS3; OCTS3NTH1nth (E.coli endonuclease III)-like 1 NTHL1 FAP3, NTH1, OCTS3, hNTH1 nth like DNA glycosylase 1 endonuclease III-like protein 1|DNA glycoslyase/AP lyase|DNA glycosylase/AP lyase|bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase|bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase|nth endonuclease III-like 1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on NTHL1, check out the NTHL1 Infographic

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In this infographic, you will see the following information for NTHL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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