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Product Info Summary
| SKU: | LS054681 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1 |
Product info
Product Name
PHD4 (P4HTM) (NM_177939) Human Over-expression Lysate
See all PHD4/HIF Prolyl Hydroxylase 4 products
SKU/Catalog Number
LS054681
Size
100 µg
Description
Transient overexpression lysate of prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4HTM), transcript variant 1
Storage & Handling
Cite This Product
PHD4 (P4HTM) (NM_177939) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS054681)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PHD4 (P4HTM) Human Over-expression Lysates NM_177939.
Western blot validation of overexpression lysate (LS054681) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with P4HTM (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for P4HTM (Source: Uniprot.org, NCBI)
Gene Name
P4HTM
Full Name
Transmembrane prolyl 4-hydroxylase
Weight
56.661kDa
Alternative Names
EC 1.14.11.-; EGLN4; HIFPH4; HIF-PH4; HIF-prolyl hydroxylase 4; HPH-4; hypoxia-inducible factor prolyl 4-hydroxylase; Hypoxia-inducible factor prolyl hydroxylase 4; P4H with transmembrane domain; P4H-TMFLJ20262; PH4 hypoxia inducible factor prolyl 4 hydroxylase; PH-4; PHD4; proline 4-hydroxylase; prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum); Prolyl hydroxlase domain-containing 4; transmembrane prolyl 4-hydroxylase P4HTM EGLN4, HIDEA, HIFPH4, P4H-TM, PH-4, PH4, PHD4 prolyl 4-hydroxylase, transmembrane transmembrane prolyl 4-hydroxylase|HIF-PH4|HIF-prolyl hydroxylase 4|HPH-4|P4H with transmembrane domain|Prolyl hydroxlase domain-containing 4|hypoxia-inducible factor prolyl 4-hydroxylase|hypoxia-inducible factor prolyl hydroxylase 4|proline 4-hydroxylase|prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on P4HTM, check out the P4HTM Infographic
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In this infographic, you will see the following information for P4HTM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PHD4 (P4HTM) (NM_177939) Human Over-expression Lysate (LS054681)
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