P4HTM Antibodies

AND P4HTM ELISA kits, P4HTM Proteins

Many biological assays use antibodies to detect P4HTM, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with P4HTM in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop P4HTM antibodies...

We validate the specificity of these antibodies to P4HTM by testing them on tissues known to express P4HTM positively and negatively. Browse below to find the P4HTM antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Previous: Septin9 Next: MANSC1

P4HTM Infographic by Boster Bio

All P4HTM Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-HIF Prolyl Hydroxylases Rabbit Monoclonal Antibody

Western blot analysis of HIF Prolyl Hydroxylases expression in HeLa cell lysate

Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution

Human

Flow Cytometry, IP, IHC, WB

$370
Cat # M12260
100 μl/vial

PHD4 (P4HTM) (NM_177939) Human Over-expression Lysate

Human

$960
Cat # LS054681
20 µg, 100 µg

P4HTM Overview

Facts about Transmembrane prolyl 4-hydroxylase.

P4HTM 3D structure. Source: alphafold

Transmembrane prolyl 4-hydroxylase (P4HTM)

Catalyzes the post-translational formation of 4- hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at'Pro-402' and'Pro-564'.

May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. .

Gene Information

Human
Gene Name:	P4HTM
Uniprot:	Q9NXG6
Entrez:	54681

Family

Belongs to:

No superfamily

Alternative Names

EC 1.14.11.-; EGLN4; HIFPH4; HIF-PH4; HIF-prolyl hydroxylase 4; HPH-4; hypoxia-inducible factor prolyl 4-hydroxylase; Hypoxia-inducible factor prolyl hydroxylase 4; P4H with transmembrane domain; P4H-TMFLJ20262; PH4 hypoxia inducible factor prolyl 4 hydroxylase; PH-4; PHD4; proline 4-hydroxylase; prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum); Prolyl hydroxlase domain-containing 4; transmembrane prolyl 4-hydroxylase

Molecular Weight

Mass (kDA):
56.661 kDA

Genomic Context


Human

Location:	3p21.31\|3p21.3
Sequence:	3; NC_000003.12 (48989908..49007153)

Tissue Specificity

Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts.

Subcellular Localizations

Endoplasmic reticulum membrane; Single-pass type II membrane protein.

Diseases

Rhabdomyosarcoma, Embryonal
Cell Transformation, Neoplastic
Alveolar Rhabdomyosarcoma
Childhood Rhabdomyosarcoma
Neoplasms
Rhabdomyosarcoma
Cytogenetic Abnormality

Sarcoma

Pathways

Methylation
Tissue Development
Dna Hypermethylation
Dna Methylation
Pathogenesis
Oncogenesis

PTMs

Methylation

Research Areas

Cancer
Hypoxia
Lipid and Metabolism

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/P4HTM

References

PMID: 12163023 by Oehme F., et al. Overexpression of PH-4, a novel putative proline 4-hydroxylase, modulates activity of hypoxia-inducible transcription factors.

PMID: 17726031 by Koivunen P., et al. An endoplasmic reticulum transmembrane prolyl 4-hydroxylase is induced by hypoxia and acts on hypoxia-inducible factor alpha.