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Product Info Summary
| SKU: | LS051177 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of pleckstrin homology domain containing, family O member 1 (PLEKHO1) |
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Product info
Product Name
PLEKHO1 (NM_016274) Human Over-expression Lysate
SKU/Catalog Number
LS051177
Size
100 µg
Description
Transient overexpression lysate of pleckstrin homology domain containing, family O member 1 (PLEKHO1)
Storage & Handling
Cite This Product
PLEKHO1 (NM_016274) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS051177)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PLEKHO1 Human Over-expression Lysates NM_016274.
Western blot validation of overexpression lysate (LS051177) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PLEKHO1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PLEKHO1 (Source: Uniprot.org, NCBI)
Gene Name
PLEKHO1
Full Name
Pleckstrin homology domain-containing family O member 1
Weight
46.237kDa
Alternative Names
2810052M02Rik; Casein kinase 2-interacting protein 1; C-Jun-binding protein; CK2 interacting protein 1; HQ0024c protein; CK2-interacting protein 1; CKIP1; CKIP-1PH domain-containing family O member 1; JBP; OC120; Osteoclast maturation-associated gene 120 protein; pleckstrin homology domain containing, family O member 1; pleckstrin homology domain-containing family O member 1 PLEKHO1 CKIP-1, CKIP1, JBP, OC120 pleckstrin homology domain containing O1 pleckstrin homology domain-containing family O member 1|C-Jun-binding protein|CK2 interacting protein 1; HQ0024c protein|CK2-interacting protein 1|PH domain-containing family O member 1|casein kinase 2-interacting protein 1|osteoclast maturation-associated gene 120 protein
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PLEKHO1, check out the PLEKHO1 Infographic
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In this infographic, you will see the following information for PLEKHO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PLEKHO1 (NM_016274) Human Over-expression Lysate (LS051177)
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No publications found for LS051177
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