Click image to see more details
Product Info Summary
| SKU: | LS008493 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of protein phosphatase 1D magnesium-dependent, delta isoform (PPM1D) |
Customers Who Bought This Also Bought
Product info
Product Name
PPM1D (NM_003620) Human Over-expression Lysate
SKU/Catalog Number
LS008493
Size
100 µg
Description
Transient overexpression lysate of protein phosphatase 1D magnesium-dependent, delta isoform (PPM1D)
Storage & Handling
Cite This Product
PPM1D (NM_003620) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS008493)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
PPM1D Human Over-expression Lysates NM_003620.
Western blot validation of overexpression lysate (LS008493) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PPM1D (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PPM1D (Source: Uniprot.org, NCBI)
Gene Name
PPM1D
Full Name
Protein phosphatase 1D
Weight
66.675kDa
Superfamily
PP2C family
Alternative Names
EC 3.1.3.16; p53-induced protein phosphatase 1; PP2C-delta; PPM1D; protein phosphatase 1D magnesium-dependent, delta isoform; protein phosphatase 1D; protein phosphatase 2C delta isoform; Protein phosphatase 2C isoform delta; Protein phosphatase magnesium-dependent 1 delta; protein phosphatase, Mg2+/Mn2+ dependent, 1D; wild-type p53-induced phosphatase 1; Wip1; WIP1protein phosphatase Wip1 PPM1D IDDGIP, JDVS, PP2C-DELTA, WIP1 protein phosphatase, Mg2+/Mn2+ dependent 1D protein phosphatase 1D|protein phosphatase Wip1|wild-type p53-induced phosphatase 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PPM1D, check out the PPM1D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPM1D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PPM1D (NM_003620) Human Over-expression Lysate (LS008493)
Hello CJ!
No publications found for LS008493
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful:
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used PPM1D (NM_003620) Human Over-expression Lysate?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review PPM1D (NM_003620) Human Over-expression Lysate
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
