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Product Info Summary
| SKU: | LS023532 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 1 |
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Product info
Product Name
PRAME (NM_006115) Human Over-expression Lysate
SKU/Catalog Number
LS023532
Size
100 µg
Description
Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 1
Storage & Handling
Cite This Product
PRAME (NM_006115) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS023532)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PRAME Human Over-expression Lysates NM_006115.
Western blot validation of overexpression lysate (LS023532) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PRAME (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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PRAME Human Over-expression Lysates NM_206953.
Western blot validation of overexpression lysate (LS023532) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PRAME (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
PRAME Human Over-expression Lysates NM_206954.
Western blot validation of overexpression lysate (LS023532) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PRAME (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
PRAME Human Over-expression Lysates NM_206955.
Western blot validation of overexpression lysate (LS023532) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PRAME (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
PRAME Human Over-expression Lysates NM_206956.
Western blot validation of overexpression lysate (LS023532) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PRAME (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PRAME (Source: Uniprot.org, NCBI)
Gene Name
PRAME
Full Name
Melanoma antigen preferentially expressed in tumors
Weight
57.89kDa
Superfamily
PRAME family
Alternative Names
cancer/testis antigen 130; MAPEOIP4CT130; melanoma antigen preferentially expressed in tumors; OIP-4; Opa-interacting protein 4; Opa-interacting protein OIP4; preferentially expressed antigen in melanoma; Preferentially expressed antigen of melanoma PRAME CT130, MAPE, OIP-4, OIP4 PRAME nuclear receptor transcriptional regulator melanoma antigen preferentially expressed in tumors|Opa-interacting protein OIP4|cancer/testis antigen 130|opa-interacting protein 4|preferentially expressed antigen in melanoma|preferentially expressed antigen of melanoma
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PRAME, check out the PRAME Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRAME: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PRAME (NM_006115) Human Over-expression Lysate (LS023532)
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