PRAME Antibodies

AND PRAME ELISA kits, PRAME Proteins

Many biological assays use antibodies to detect PRAME, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with PRAME in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop PRAME antibodies...

We validate the specificity of these antibodies to PRAME by testing them on tissues known to express PRAME positively and negatively. Browse below to find the PRAME antibody that suites your experiment. We have 13 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for PRAME? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the PRAME Western Blot Guide

Designing an IHC experiment for PRAME? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the PRAME IHC Guide

PRAME Infographic by Boster Bio

All PRAME Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-PRAME Picoband® Antibody

Western blot analysis of PRAME using anti-PRAME antibody (A06628-2)

Human

ELISA, WB

$370
Cat # A06628-2
100 μg/vial

Anti-MAPE Antibody (C-term)

Overlay histogram showing Hela cells stained with A06628-1 (green line)

Human

Flow Cytometry, IHC-P, WB

$299
Cat # A06628-1
80 µl

PRAME (NM_006115) Human Over-expression Lysate

PRAME Human Over-expression Lysates NM_006115

Human

$628
Cat # LS023532
20 µg, 100 µg

Anti-PRAME Reference Antibody (Eureka patent anti-PRAME)

The purity of Anti-PRAME Reference Antibody (Eureka patent anti-PRAME)is more than 95%

Human, Mouse

ELISA, Flow Cytometry, Functional Assay, Kinetics

$486
Cat # M06628
100 μg

PRAMEF16 (NM_001045480) Human Over-expression Lysate

Human

$960
Cat # LS654348
20 µg, 100 µg

PRAMEF7 (NM_001012277) Human Over-expression Lysate

Human

$960
Cat # LS441871
20 µg, 100 µg

PRAMEF3 (NM_001013692) Human Over-expression Lysate

Human

$960
Cat # LS401940
20 µg, 100 µg

PRAMEF18 (NM_001099850) Human Over-expression Lysate

Human

$960
Cat # LS391003
20 µg, 100 µg

PRAMEF8 (NM_001012276) Human Over-expression Lysate

Human

$960
Cat # LS391002
20 µg, 100 µg

PRAMEF12 (NM_001080830) Human Over-expression Lysate

Human

$960
Cat # LS390999
20 µg, 100 µg

PRAMEF10 (NM_001039361) Human Over-expression Lysate

Human

$628
Cat # LS343071
20 µg, 100 µg

PRAMEF5 (NM_001013407) Human Over-expression Lysate

Human

$960
Cat # LS343068
20 µg, 100 µg

PRAMEF19 (NM_001099790) Human Recombinant Protein

Coomassie blue staining of purified PRAMEF19 protein

$1249
Cat # PROTQ5SWL8
20 µg

PRAME Overview

Facts about Melanoma antigen preferentially expressed in tumors.

PRAME 3D structure. Source: alphafold

Melanoma antigen preferentially expressed in tumors (PRAME)

Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.

Gene Information

Human
Gene Name:	PRAME
Uniprot:	P78395
Entrez:	23532

Family

Belongs to:

PRAME family

Alternative Names

cancer/testis antigen 130; MAPEOIP4CT130; melanoma antigen preferentially expressed in tumors; OIP-4; Opa-interacting protein 4; Opa-interacting protein OIP4; preferentially expressed antigen in melanoma; Preferentially expressed antigen of melanoma

Molecular Weight

Mass (kDA):
57.89 kDA

Genomic Context


Human

Location:	22q11.22
Sequence:	22; NC_000022.11 (22547696..22559361, complement)

Tissue Specificity

Expressed in testis. Detected in samples of kidney, brain and skin.

Subcellular Localizations

Nucleus. Cell membrane.

Diseases

Neoplasms
Leukemia
Malignant Neoplasms
Melanoma
Myeloid Leukemia
Leukemia, Myelocytic, Acute
Neoplasm, Residual
Myeloid Leukemia, Chronic
Carcinoma
Malignant Paraganglionic Neoplasm
Hematologic Neoplasms
Acute Lymphocytic Leukemia
Lymphoma

Neoplasm Metastasis
Acute Leukemia
Lymphoid Leukemia
Chronic Lymphocytic Leukemia
Aortic Aneurysm, Thoracic
Skin Neoplasms
Blast Phase

Pathways

Immune Response
Cell Cycle
Methylation
Cell Cycle Arrest
Dna Methylation
Pathogenesis
Reverse Transcription
Cell Proliferation
Melanocyte Differentiation
Spermatogenesis
Rna Interference
Cell Adhesion
Transposition

Stem Cell Differentiation
Protein Unfolding
Chromosome Segregation
Cell Differentiation
Detection Of Tumor Cell
Phototransduction
Oogenesis

PTMs

Methylation

Demethylation

Myristoylation

Research Areas

Cancer

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PRAME

References

PMID: 9047241 by Ikeda H., et al. Characterization of an antigen that is recognized on a melanoma showing partial HLA loss by CTL expressing an NK inhibitory receptor.

PMID: 9466265 by Williams J.M., et al. Using the yeast two-hybrid system to identify human epithelial cell proteins that bind gonococcal Opa proteins: intracellular gonococci bind pyruvate kinase via their Opa proteins and require host pyruvate for growth.