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Product Info Summary
| SKU: | LS029964 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of prickle homolog 4 (Drosophila) (PRICKLE4) |
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Product info
Product Name
PRICKLE4 (NM_013397) Human Over-expression Lysate
SKU/Catalog Number
LS029964
Size
100 µg
Description
Transient overexpression lysate of prickle homolog 4 (Drosophila) (PRICKLE4)
Storage & Handling
Cite This Product
PRICKLE4 (NM_013397) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS029964)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PRICKLE4 Human Over-expression Lysates NM_013397.
Western blot validation of overexpression lysate (LS029964) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PRICKLE4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PRICKLE4 (Source: Uniprot.org, NCBI)
Gene Name
PRICKLE4
Full Name
Prickle-like protein 4
Weight
37.551kDa
Superfamily
prickle / espinas / testin family
Alternative Names
over-expressed breast tumor protein; prickle homolog 4 (Drosophila); prickle-like protein 4 PRICKLE4 C6orf49, OBTP, OEBT, TOMM6 prickle planar cell polarity protein 4 prickle-like protein 4|over-expressed breast tumor protein|overexpressed breast tumor protein|overexpressed breast tumour protein|prickle homolog 4|translocase of outer mitochondrial membrane 6 homolog
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PRICKLE4, check out the PRICKLE4 Infographic
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In this infographic, you will see the following information for PRICKLE4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PRICKLE4 (NM_013397) Human Over-expression Lysate (LS029964)
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No publications found for LS029964
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