Anti-Prickle-like protein 4 PRICKLE4 Antibody

Product Name

Anti-Prickle-like protein 4 PRICKLE4 Antibody

View all PRICKLE4 Antibodies

SKU/Catalog Number

A14637

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Prickle-like protein 4 PRICKLE4 Antibody (Catalog # A14637). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.

Storage & Handling

PRICKLE4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Prickle-like protein 4 PRICKLE4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14637)

Host

Rabbit

Contents

PRICKLE4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PRICKLE4 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PRICKLE4. The immunogen is located within the first 50 amino acids of PRICKLE4.

Cross-reactivity

Two isoforms of PRICKLE4 are known to exist. PRICKLE4 antibody is predicted to not cross-react with other PRICKLE protein family members.

Reactive Species

A14637 is reactive to PRICKLE4 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

37.6 kDa

Background of PRICKLE4

PRICKLE4, also known as over-expressed breast tumor protein (OBTP), is a member of the LIM family of proteins. It possesses two LIM domains as well as a PET domain and is thought to localize to the nucleus. Its expression in multiple malignant tissue differentiations indicate that it may play a role in cancer differentiation. Two other members of the LIM family, PRICKLE1 and PRICKLE2, are thought to function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity, suggesting the PRICKLE4 may play a similar role.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A14637 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PRICKLE4 antibody can be used for detection of PRICKLE4 by Western blot at 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information PRICKLE4

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