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Product Info Summary
| SKU: | LS005500 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of protein phosphatase 1, catalytic subunit, beta isoform (PPP1CB), transcript variant 1 |
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Product info
Product Name
Protein Phosphatase 1 beta (PPP1CB) (NM_002709) Human Over-expression Lysate
See all Protein Phosphatase 1 beta products
SKU/Catalog Number
LS005500
Size
100 µg
Description
Transient overexpression lysate of protein phosphatase 1, catalytic subunit, beta isoform (PPP1CB), transcript variant 1
Storage & Handling
Cite This Product
Protein Phosphatase 1 beta (PPP1CB) (NM_002709) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005500)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Protein Phosphatase 1 beta (PPP1CB) Human Over-expression Lysates NM_002709.
Western blot validation of overexpression lysate (LS005500) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PPP1CB (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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Protein Phosphatase 1 beta (PPP1CB) Human Over-expression Lysates NM_206876.
Western blot validation of overexpression lysate (LS005500) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PPP1CB (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PPP1CB (Source: Uniprot.org, NCBI)
Gene Name
PPP1CB
Full Name
Serine/threonine-protein phosphatase PP1-beta catalytic subunit
Weight
37.187kDa
Superfamily
PPP phosphatase family
Alternative Names
EC 3.1.3.16; EC 3.1.3.53; MGC3672; PP1beta; PP-1Bprotein phosphatase 1, catalytic subunit, delta isoform; PPP1CD; protein phosphatase 1, catalytic subunit, beta isoform; protein phosphatase 1, catalytic subunit, beta isozyme; protein phosphatase 1-beta; protein phosphatase 1-delta; serine/threonine protein phosphatase PP1-beta catalytic subunit; serine/threonine-protein phosphatase PP1-beta catalytic subunit PPP1CB HEL-S-80p, MP, NSLH2, PP-1B, PP1B, PP1beta, PP1c, PPP1CD, PPP1beta protein phosphatase 1 catalytic subunit beta serine/threonine-protein phosphatase PP1-beta catalytic subunit|epididymis secretory sperm binding protein Li 80p|myosin phosphatase|protein phosphatase 1, catalytic subunit, beta isozyme|protein phosphatase 1-beta|protein phosphatase 1-delta
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PPP1CB, check out the PPP1CB Infographic
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In this infographic, you will see the following information for PPP1CB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Protein Phosphatase 1 beta (PPP1CB) (NM_002709) Human Over-expression Lysate (LS005500)
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