Product Info Summary
SKU: | LS022976 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of PAX interacting (with transcription-activation domain) protein 1 (PAXIP1) |
Product info
Product Name
PTIP (PAXIP1) (NM_007349) Human Over-expression Lysate
SKU/Catalog Number
LS022976
Size
100 µg
Description
Transient overexpression lysate of PAX interacting (with transcription-activation domain) protein 1 (PAXIP1)
Storage & Handling
Cite This Product
PTIP (PAXIP1) (NM_007349) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS022976)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Protein Target Info & Infographic
Gene/Protein Information for PAXIP1 (Source: Uniprot.org, NCBI)
Gene Name
PAXIP1
Full Name
PAX-interacting protein 1
Weight
121.341kDa
Alternative Names
CAGF28; PACIP1; PAX interacting (with transcription-activation domain) protein 1; PAX transactivation activation domain-interacting protein; PAX transcription activation domain interacting protein 1 like; PAX-interacting protein 1; PAXIP1LCAGF29; protein encoded by CAG trinucleotide repeats; PTIPFLJ41049; TNRC2 PAXIP1 CAGF28, CAGF29, PACIP1L, PTIP, TNRC2, PAXIP1 PAX interacting protein 1 PAX-interacting protein 1|PAX interacting (with transcription-activation domain) protein 1|PAX transcription activation domain interacting protein 1 like|protein encoded by CAG trinucleotide repeats
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PAXIP1, check out the PAXIP1 Infographic
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In this infographic, you will see the following information for PAXIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PTIP (PAXIP1) (NM_007349) Human Over-expression Lysate (LS022976)
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No publications found for LS022976
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