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Product Info Summary
| SKU: | EK0303 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. |
Product info
Product Name
Rat Activin A ELISA Kit PicoKine®
SKU/Catalog Number
EK0303
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat Activin A ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Inhba in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 12pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Activin A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0303)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: G311-S426
Sensitivity
<12 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0303 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0303 is reactive to INHBA in Rat samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.
Application Guarantee
EK0303 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Activin A
Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0303-CAP | Anti-Rat INHBA Pre-coated 96-well strip microplate | 1 |
| EK0303-ST | Rat INHBA Standard | 2 vials, 10 ng/tube |
| EK0303-DA | Rat INHBA Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.025 | 0.081 | 0.120 | 0.222 | 0.433 | 0.761 | 1.429 | 2.365 |
Data Example Images
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Rat Activin A PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Activin A, For serum, dilution ratio is 1:1 and concentration is 87-1759 pg/ml.
For plasma, dilution ratio is 1:1 and concentration is 78-374 pg/ml..
Some articles we found to cite concentrations of Activin A in samples: 15070852 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Activin A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 31 | 151 | 450 | 28 | 138 | 453 |
| Standard deviation | 1.30 | 8.00 | 24.20 | 1.56 | 8.69 | 25.36 |
| CV (%) | 4.2% | 5.3% | 5.6% | 5.6% | 6.3% | 5.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Activin A in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 31 | 31 | 27 | 31 | 30 | 1.73 | 5.7% |
| Sample 2 | 151 | 165 | 144 | 171 | 157 | 10.75 | 6.8% |
| Sample 3 | 450 | 412 | 404 | 462 | 432 | 24.53 | 5.6% |
Protein Target Info & Infographic
Gene/Protein Information For INHBA (Source: Uniprot.Org, NCBI)
Gene Name
INHBA
Full Name
Inhibin beta A chain
Weight
47.442kDa
Superfamily
TGF-beta family
Alternative Names
Activin A; activin AB alpha polypeptide; Activin beta-A chain; erythroid differentiation factor; Erythroid differentiation protein; follicle-stimulating hormone-releasing protein; FSH-releasing protein; inhibin beta A chain; inhibin beta A subunit; Inhibin, beta-1 INHBA EDF, FRP inhibin subunit beta A inhibin beta A chain|FSH-releasing protein|Inhibin, beta-1|activin beta-A chain|erythroid differentiation factor|erythroid differentiation protein|follicle-stimulating hormone-releasing protein|inhibin beta A subunit|inhibin, beta A (activin A, activin AB alpha polypeptide)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on INHBA, check out the INHBA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for INHBA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat Activin A ELISA Kit PicoKine® (EK0303)
Hello CJ!
EK0303 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Changes in the reproductive endocrine function in rat following intraovary microinjection of inhba overexpression lentivirus vectors
The Extracts of Pacific Oyster (Crassostrea Gigas) Alleviate Ovarian Functional Disorders of Female Rats with Exposure to Bisphenol a Through Decreasing FSHR Expression in Ovarian Tissues
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Rat Activin A ELISA Kit PicoKine®
Question
Q: is it okay to use citrate plasma as samples in Rat Activin A Picokine® ELISA Kit (Catalog # EK0303)?
C. Lewis
Verified customer
Asked: 2020-01-30
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Activin A causing disruption of its protein structure. Activin A may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Activin A ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-01-30
Question
Do you sell the wells/strips from EK0303 Elisa kit separately without the buffers?
Verified customer
Asked: 2020-01-14
Answer
We sell the complete kit. The only thing that gets sold separately if needed are the standards. We don't really disintegrate our kits and sell as it requires additional time and effort to do that. Also, the components to be saved are of no use to us as they are from particular lot numbers.
Boster Scientific Support
Answered: 2020-01-14
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-06-08
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-06-08
Question
Q: how to analyze ELISA data? I measured Activin A level in saliva.
Verified Customer
Verified customer
Asked: 2018-05-06
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-05-06
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-09-24
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-09-24
Question
Q: What is the optimal O.D. value for Activin A ELISA kit? I used your Activin A ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Activin A even though the O.D. values are not very high?
R. Wu
Verified customer
Asked: 2016-01-27
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Activin A ELISA kit to have sensitivity of 12pg/ml, that means the minimum amount of Activin A that can be declared/interpreted as positive by the above standard is 12pg/ml.
Boster Scientific Support
Answered: 2016-01-27
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Activin A using the EK0303 Rat Activin A Picokine® ELISA Kit?
L. Anand
Verified customer
Asked: 2015-03-31
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2015-03-31
