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Product Info Summary
| SKU: | EK1267 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, tissue, serum and plasma (heparin or EDTA) |
Product info
Product Name
Rat Myostatin/GDF8 ELISA Kit PicoKine®
View all GDF-8/Myostatin ELISA kits
SKU/Catalog Number
EK1267
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat Myostatin/GDF8 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat MSTN in cell culture supernatants, tissue, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Myostatin/GDF8 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1267)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: D268-S376
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1267 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1267 is reactive to MSTN in Rat samples
Validated Sample Types
cell culture supernatants, tissue, serum and plasma (heparin or EDTA)
Application Guarantee
EK1267 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GDF-8/Myostatin
Myostatin (also known as growth differentiation factor 8, abbreviated GDF-8) is a myokine, a protein produced and released by myocytes that acts on muscle cells' autocrine function to inhibit myogenesis: muscle cell growth and differentiation. In humans it is encoded by the MSTN gene. It is mapped to 9q22. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1267-CAP | Anti-Rat MSTN Pre-coated 96-well strip microplate | 1 |
| EK1267-ST | Rat MSTN Standard | 2 vials, 10 ng/tube |
| EK1267-DA | Rat MSTN Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.076 | 0.150 | 0.185 | 0.292 | 0.520 | 0.839 | 1.496 | 2.292 |
Data Example Images
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Rat Myostatin/GDF8 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect GDF-8/Myostatin, Dilution ratio of 1:10, concentration in serum and plasma is around 5 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Myostatin/GDF8 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 55 | 228 | 845 | 60 | 239 | 884 |
| Standard deviation | 2.31 | 14.36 | 38.03 | 3.18 | 15.30 | 63.65 |
| CV (%) | 4.2% | 6.3% | 4.5% | 5.3% | 6.4% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of GDF-8/Myostatin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 55 | 53 | 50 | 52 | 52 | 1.80 | 3.4% |
| Sample 2 | 228 | 261 | 228 | 264 | 245 | 17.28 | 7.0% |
| Sample 3 | 845 | 788 | 838 | 845 | 829 | 23.84 | 2.8% |
Protein Target Info & Infographic
Gene/Protein Information For MSTN (Source: Uniprot.Org, NCBI)
Gene Name
MSTN
Full Name
Growth/differentiation factor 8
Weight
42.75kDa
Superfamily
TGF-beta family
Alternative Names
GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSLHP; MSTN; Myostatin MSTN GDF8, MSLHP myostatin growth/differentiation factor 8
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MSTN, check out the MSTN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MSTN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat Myostatin/GDF8 ELISA Kit PicoKine® (EK1267)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Rat Myostatin/GDF8 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for GDF-8 ELISA kit? I performed your GDF-8 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain GDF-8 even though the O.D. values are not very high?
J. Collins
Verified customer
Asked: 2020-02-04
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this GDF-8 ELISA kit to have sensitivity of <.10pg/ml, that means the minimum amount of GDF-8 that can be declared/interpreted as positive by the above standard is <.10pg/ml.
Boster Scientific Support
Answered: 2020-02-04
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis GDF-8 using the EK1267 Rat GDF-8 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-06-07
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-03-09
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-03-09
Question
Q: is it okay to use citrate plasma as samples in Rat GDF-8 Picokine® ELISA Kit (Catalog # EK1267)?
L. Banerjee
Verified customer
Asked: 2018-12-31
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of GDF-8 causing degradation of its protein structure. GDF-8 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the GDF-8 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-12-31
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
H. King
Verified customer
Asked: 2018-12-08
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-12-08
