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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, tissue, serum and plasma (heparin or EDTA)|
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Human Myostatin/GDF8 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Myostatin/GDF8 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MSTN in cell culture supernatants, tissue, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Myostatin/GDF8 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1265)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: D267-S375
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1265 is reactive to MSTN in Human samples
Validated Sample Types
cell culture supernatants, tissue, serum and plasma (heparin or EDTA)
EK1265 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GDF-8/Myostatin
Myostatin (also known as growth differentiation factor 8, abbreviated GDF-8) is a myokine, a protein produced and released by myocytes that acts on muscle cells' autocrine function to inhibit myogenesis: muscle cell growth and differentiation. In humans it is encoded by the MSTN gene. It is mapped to 2q32.2. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals.
|EK1265-CAP||Anti-Human MSTN Pre-coated 96-well strip microplate||1|
|EK1265-ST||Human MSTN Standard||2 vials, 10 ng/tube|
|EK1265-DA||Human MSTN Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Myostatin/GDF8 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect GDF-8/Myostatin, Dilution ratio of 1:1, concentration in serum is around 500pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myostatin/GDF8 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of GDF-8/Myostatin in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For MSTN (Source: Uniprot.Org, NCBI)
Growth/differentiation factor 8
GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSLHP; MSTN; Myostatin MSTN GDF8, MSLHP myostatin growth/differentiation factor 8*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MSTN, check out the MSTN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MSTN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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