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Product Info Summary
| SKU: | LS011236 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of ring finger protein 139 (RNF139) |
Product info
Product Name
RNF139 (NM_007218) Human Over-expression Lysate
SKU/Catalog Number
LS011236
Size
100 µg
Description
Transient overexpression lysate of ring finger protein 139 (RNF139)
Storage & Handling
Cite This Product
RNF139 (NM_007218) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS011236)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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RNF139 Human Over-expression Lysates NM_007218.
Western blot validation of overexpression lysate (LS011236) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RNF139 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for RNF139 (Source: Uniprot.org, NCBI)
Gene Name
RNF139
Full Name
E3 ubiquitin-protein ligase RNF139
Weight
75.994kDa
Alternative Names
EC 6.3.2.-; HRCA1; patched related protein translocated in renal cancer; RCA1multiple membrane spanning receptor TRC8; ring finger protein 139MGC31961; Translocation in renal carcinoma on chromosome 8 protein; TRC8E3 ubiquitin-protein ligase RNF139 RNF139 HRCA1, RCA1, TRC8 ring finger protein 139 E3 ubiquitin-protein ligase RNF139|RING-type E3 ubiquitin transferase RNF139|multiple membrane spanning receptor TRC8|patched related protein translocated in renal cancer|translocation in renal carcinoma on chromosome 8 protein|translocation in renal carcinoma, chromosome 8
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on RNF139, check out the RNF139 Infographic
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In this infographic, you will see the following information for RNF139: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For RNF139 (NM_007218) Human Over-expression Lysate (LS011236)
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No publications found for LS011236
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